{"id":"W1001608507","doi":"10.2144/00296bm07","title":"5′RACE by Tailing a General Template-Switching Oligonucleotide","year":2000,"lang":"en","type":"article","venue":"BioTechniques","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada; University of Saskatchewan","keywords":"Biology; Oligonucleotide; DNA origami; Molecular biology; Genetics; Computational biology; DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001169428,0.002435885,0.00237102,0.001103599,0.0007601018,0.0008517429,0.00146839,0.0009607075,0.02343951],"category_scores_gemma":[0.001070029,0.002234513,0.002393647,0.0006109995,0.0007712789,0.0005897198,0.001092359,0.003971417,0.01910249],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006892081,"about_ca_system_score_gemma":0.001087742,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006703173,"about_ca_topic_score_gemma":0.001793459,"domain_scores_codex":[0.998583,0.0002216752,0.000164362,0.0006333395,0.0001516611,0.0002459461],"domain_scores_gemma":[0.9993398,0.0002139707,0.00005300467,0.0002281076,0.00009533791,0.00006983118],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003961652,0.0002000666,0.000287832,0.0003944803,0.00004452431,0.0001350938,0.0001459178,0.0003218095,0.9756349,0.002073501,0.006203002,0.01416284],"study_design_scores_gemma":[0.0002242953,0.0006163529,0.001738555,0.00008982424,0.0002046763,0.0004974875,0.00004562371,0.002891646,0.8479657,0.001264695,0.1443656,0.00009561749],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1846356,0.004222705,0.7165135,0.001379918,0.002772758,0.005371741,0.03282921,0.01895536,0.03331919],"genre_scores_gemma":[0.2321893,0.003283449,0.5443515,0.002332439,0.0003992236,0.005529725,0.1213175,0.005261834,0.08533507],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.02343951,"threshold_uncertainty_score":0.07841295,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005531173933411637,"score_gpt":0.2580151141608922,"score_spread":0.2524839402274806,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}