{"id":"W112150230","doi":"10.1016/bs.mie.2015.01.005","title":"Rapid and Facile Recombinant Expression of Bovine Rhodopsin in HEK293S GnTI− Cells Using a PiggyBac Inducible System","year":2015,"lang":"en","type":"article","venue":"Methods in enzymology on CD-ROM/Methods in enzymology","topic":"Photoreceptor and optogenetics research","field":"Neuroscience","cited_by":14,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"National Institutes of Health; National Eye Institute; Canada Excellence Research Chairs, Government of Canada; Canada Research Chairs","keywords":"Rhodopsin; G protein-coupled receptor; HEK 293 cells; Biology; Drosophila melanogaster; Cell biology; Mutant; Schneider 2 cells; Molecular biology; Mutagenesis; Biochemistry; Receptor; Gene; Signal transduction; Retinal; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaresearch","metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.01988574,0.0006976856,0.002071051,0.002599173,0.0001230138,0.00003910825,0.0011692,0.001254601,0.0001330285],"category_scores_gemma":[0.009005749,0.0006709878,0.0001539053,0.002220951,0.00119249,0.0002054735,0.001027136,0.001950109,0.00001538703],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006758011,"about_ca_system_score_gemma":0.0004011673,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009444461,"about_ca_topic_score_gemma":0.0002129072,"domain_scores_codex":[0.9616516,0.03210691,0.001978755,0.002054183,0.0005475479,0.001661072],"domain_scores_gemma":[0.9890931,0.008504809,0.000587442,0.001271428,0.0001468534,0.0003964247],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009676585,0.0006431784,0.005507495,0.0001819497,0.000008692773,0.0003312888,0.001560484,0.0006870826,0.9726794,0.0009175932,0.00009624081,0.01641889],"study_design_scores_gemma":[0.003529917,0.001716936,0.006336561,0.0003056922,0.00001532836,0.0003995638,0.0008039586,0.003222681,0.976994,0.004803174,0.001264192,0.0006080031],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9732211,0.00123172,0.01902779,0.0000835613,0.002966924,0.001174431,0.00003277393,0.00007168756,0.002190078],"genre_scores_gemma":[0.4246345,0.0002572256,0.5745302,0.0001552496,0.00005465726,0.0001380432,0.000003728329,0.00007745984,0.0001489989],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.5555024,"threshold_uncertainty_score":0.9995741,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.2353500990963753,"score_gpt":0.4660879173441496,"score_spread":0.2307378182477743,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}