{"id":"W116712366","doi":"10.1007/7651_2015_212","title":"A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells","year":2015,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":false,"ca_institutions":"Lunenfeld-Tanenbaum Research Institute","funders":"","keywords":"Embryonic stem cell; Stem cell; Small interfering RNA; Limiting; Regenerative medicine; Biology; Cell biology; Gene; Computational biology; RNA; Genetics; Engineering","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001396409,0.002663464,0.001769254,0.002594178,0.002123243,0.0007681539,0.00218765,0.001400783,0.02083577],"category_scores_gemma":[0.0009639004,0.002137354,0.002066271,0.001914679,0.0006725329,0.0006717044,0.0009123624,0.003717616,0.02432441],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009384351,"about_ca_system_score_gemma":0.001830412,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001771766,"about_ca_topic_score_gemma":0.005488119,"domain_scores_codex":[0.9984214,0.0003288159,0.0003088421,0.0002688948,0.0004408973,0.0002311943],"domain_scores_gemma":[0.9993145,0.00022036,0.00005290112,0.0001891054,0.0001598434,0.00006328254],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000168347,0.0001612898,0.00009008742,0.0008532203,0.00006377235,0.0006145055,0.0001612063,0.0006198286,0.9611733,0.003325148,0.01463085,0.01813851],"study_design_scores_gemma":[0.0002155543,0.0005442117,0.001672818,0.000184021,0.0001667833,0.001605086,0.00005668037,0.002401276,0.6614133,0.001893254,0.329681,0.0001661111],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.02174797,0.008900119,0.8787569,0.001167752,0.002093793,0.01196636,0.02663887,0.01199903,0.03672927],"genre_scores_gemma":[0.07637484,0.01438992,0.7126036,0.001586734,0.0004525397,0.04146221,0.07217597,0.002450922,0.07850337],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.02083577,"threshold_uncertainty_score":0.06970263,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06776083671774935,"score_gpt":0.4510054275727836,"score_spread":0.3832445908550343,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}