{"id":"W1202517771","doi":"10.1016/j.ymeth.2015.08.011","title":"RGS proteins destroy spare receptors: Effects of GPCR-interacting proteins and signal deamplification on measurements of GPCR agonist potency","year":2015,"lang":"en","type":"review","venue":"Methods","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":false,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"G protein-coupled receptor; Functional selectivity; Receptor; Heterotrimeric G protein; Signal transduction; Cell biology; G protein; Agonist; Biology; Ligand (biochemistry); Chemistry; Second messenger system; Biophysics; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0023347,0.0004738491,0.001252087,0.0001361412,0.00008280338,0.00003296746,0.000321698,0.0004845121,0.00003003467],"category_scores_gemma":[0.0009554256,0.000395002,0.0003346689,0.0001673363,0.00007104129,0.000007660189,0.000135971,0.000292892,0.000002437653],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006833332,"about_ca_system_score_gemma":0.000290519,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005881374,"about_ca_topic_score_gemma":0.000002541963,"domain_scores_codex":[0.9963621,0.001486155,0.0008316082,0.000661541,0.0003654494,0.0002930711],"domain_scores_gemma":[0.9977694,0.0001333001,0.001109193,0.0005233831,0.0003269761,0.0001377932],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003853969,0.00009265728,0.000005157756,0.006793064,0.0001646719,8.006974e-7,0.00004017166,0.000003612349,0.303276,0.00001574232,0.0000409424,0.6895286],"study_design_scores_gemma":[0.0003217294,0.001134862,0.000001696118,0.008167426,0.0003651185,0.00001189831,0.00002677795,0.000007062864,0.7628033,0.00007555869,0.226693,0.0003915129],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.0005318,0.8721079,0.1237359,0.00000555183,0.0004546288,0.002775764,0.00002897672,0.00001558153,0.0003438005],"genre_scores_gemma":[0.001315323,0.5214921,0.4750806,0.00001520954,0.0005129787,0.0007114852,0.0003936162,0.0001741603,0.0003045794],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.6891372,"threshold_uncertainty_score":0.9998502,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1104801396894526,"score_gpt":0.4064694492782405,"score_spread":0.2959893095887879,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}