{"id":"W122810863","doi":"10.1007/978-1-59745-245-8_19","title":"RNA Isolation and Real-Time Quantitative RT-PCR","year":2008,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"Children’s Health Research Institute; Lawson Health Research Institute; Western University","funders":"","keywords":"Real-time polymerase chain reaction; Isolation (microbiology); Computational biology; RNA; RNA extraction; Biology; Computer science; Microbiology; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.005557583,0.005661645,0.005694111,0.007303878,0.002764179,0.002487159,0.005351747,0.002192327,0.0171318],"category_scores_gemma":[0.00427793,0.002826812,0.00355578,0.00696193,0.003140799,0.001442349,0.001992682,0.006892477,0.01611542],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001020991,"about_ca_system_score_gemma":0.002330148,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006205557,"about_ca_topic_score_gemma":0.001764596,"domain_scores_codex":[0.9879127,0.002514658,0.002004258,0.003785974,0.002745025,0.00103723],"domain_scores_gemma":[0.9960266,0.001467123,0.0004490494,0.0008265997,0.0009943859,0.0002361614],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000609079,0.000651927,0.0004265232,0.002870945,0.0001437627,0.0004153027,0.000351221,0.001995458,0.9573852,0.003198593,0.003414269,0.02853771],"study_design_scores_gemma":[0.0005079951,0.002196893,0.002199073,0.0005604876,0.0004555591,0.0006836982,0.0002048882,0.01273647,0.7875495,0.004566133,0.1879503,0.0003890791],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.01922732,0.005171614,0.9243267,0.000442135,0.0016312,0.01602176,0.01725575,0.006932892,0.008990597],"genre_scores_gemma":[0.01644719,0.004667243,0.928836,0.0004025152,0.0005115098,0.02382772,0.01649174,0.001334748,0.007481257],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.0171318,"threshold_uncertainty_score":0.05731159,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02624009529414003,"score_gpt":0.4022161186075505,"score_spread":0.3759760233134105,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}