{"id":"W124813241","doi":"10.2144/02332ht03","title":"Nonradioactive Detection of Retroviral- Associated RNase H Activity in a Microplate-Based, High-Throughput Format","year":2002,"lang":"en","type":"article","venue":"BioTechniques","topic":"HIV/AIDS drug development and treatment","field":"Medicine","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research","keywords":"RNase H; Reverse transcriptase; Biotinylation; RNase P; Oligonucleotide; Molecular biology; Biology; RNA-Directed DNA Polymerase; DNA; High-throughput screening; Enzyme; Streptavidin; Biochemistry; RNA; Chemistry; Biotin","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003129381,0.002367751,0.001507164,0.001444958,0.0006410262,0.001335791,0.002544012,0.001193413,0.002986183],"category_scores_gemma":[0.001240509,0.0009677634,0.0007824639,0.001181126,0.0009268761,0.001078903,0.0007344256,0.002384671,0.002903931],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005673673,"about_ca_system_score_gemma":0.000708914,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004203119,"about_ca_topic_score_gemma":0.001007778,"domain_scores_codex":[0.9961522,0.00162598,0.0002116005,0.0006766473,0.001144583,0.0001888238],"domain_scores_gemma":[0.9984332,0.0008467496,0.0001589423,0.0002167934,0.0002325632,0.0001118098],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001176677,0.0001642739,0.0001742299,0.0002125957,0.0000325197,0.00004441568,0.00007499261,0.0002037647,0.9955946,0.000260432,0.0001850405,0.002935513],"study_design_scores_gemma":[0.0000335734,0.0003196076,0.0004699424,0.000008903858,0.00002582746,0.0001252889,0.00002556828,0.004119333,0.9935365,0.0001143682,0.001203894,0.00001725523],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2532792,0.003054325,0.731687,0.0003287334,0.0004096444,0.0009518614,0.001223795,0.004674414,0.004391057],"genre_scores_gemma":[0.2631995,0.004337205,0.7037207,0.0002889729,0.0002539823,0.002287788,0.002794475,0.0002839202,0.02283351],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.003129381,"threshold_uncertainty_score":0.01654994,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0159507271664171,"score_gpt":0.2404331420336668,"score_spread":0.2244824148672497,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}