{"id":"W1486262659","doi":"10.1016/s0076-6879(00)22013-2","title":"Purification and Use of Granzyme B","year":2000,"lang":"en","type":"article","venue":"Methods in enzymology on CD-ROM/Methods in enzymology","topic":"Cell death mechanisms and regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba","funders":"","keywords":"Perforin; Granzyme B; Granzyme; Cytotoxic T cell; Apoptosis; CTL*; Chemistry; Molecular biology; Caspase; Cell biology; Biology; Biochemistry; Programmed cell death","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.004008921,0.0004100895,0.0008072608,0.0005110804,0.00007703035,0.00001593912,0.0003274132,0.001137776,0.0002893979],"category_scores_gemma":[0.0009985715,0.0004044218,0.000140942,0.0004570633,0.0005497572,0.00001765514,0.000132793,0.0004836712,0.000006784731],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004745854,"about_ca_system_score_gemma":0.00006179007,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002024995,"about_ca_topic_score_gemma":0.0002914009,"domain_scores_codex":[0.992062,0.005142967,0.0009908394,0.00106335,0.0001338821,0.0006069413],"domain_scores_gemma":[0.9977347,0.0009379854,0.000275606,0.0008556662,0.00008505738,0.000110938],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004647763,0.0002788192,0.002481296,0.00003193201,0.00003796893,0.00002263412,0.0001824257,0.0007597383,0.7423285,0.005278217,0.0001043432,0.2480294],"study_design_scores_gemma":[0.004034647,0.00278792,0.4003527,0.00009213743,0.00009413526,0.0006102886,0.0001357687,0.001147541,0.4706259,0.03753049,0.08139396,0.001194579],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9184708,0.00180078,0.07700327,0.0002572993,0.000606822,0.0005778582,0.00001544016,0.00002860975,0.001239112],"genre_scores_gemma":[0.1750848,0.001567862,0.8210027,0.0004866634,0.0001154472,0.0001047578,0.00007231845,0.00005673308,0.001508714],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.7439995,"threshold_uncertainty_score":0.9998407,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04219067459534841,"score_gpt":0.3797231265398754,"score_spread":0.337532451944527,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}