{"id":"W1486262659","doi":"10.1016/s0076-6879(00)22013-2","title":"Purification and Use of Granzyme B","year":2000,"lang":"en","type":"article","venue":"Methods in enzymology on CD-ROM/Methods in enzymology","topic":"Cell death mechanisms and regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba","funders":"","keywords":"Perforin; Granzyme B; Granzyme; Cytotoxic T cell; Apoptosis; CTL*; Chemistry; Molecular biology; Caspase; Cell biology; Biology; Biochemistry; Programmed cell death","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008069571,0.001255405,0.001088412,0.001318122,0.001241173,0.0009993698,0.001574285,0.0006247421,0.004567625],"category_scores_gemma":[0.001152657,0.001040148,0.001020262,0.0009407435,0.0005998762,0.0006627397,0.001709751,0.003015422,0.01000941],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004376222,"about_ca_system_score_gemma":0.0007391945,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007901324,"about_ca_topic_score_gemma":0.0007689617,"domain_scores_codex":[0.9991118,0.0001072608,0.0001128727,0.0002570865,0.0002196743,0.0001913152],"domain_scores_gemma":[0.9992855,0.000125633,0.00004209626,0.0003000938,0.0001164519,0.0001301786],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002635521,0.0000948736,0.0002232791,0.0001010506,0.00002719847,0.00007816648,0.00007417746,0.0002085219,0.9862624,0.0005344392,0.0005749287,0.01155736],"study_design_scores_gemma":[0.0001067632,0.0001397434,0.0009590476,0.00002123004,0.00003836997,0.0003113784,0.00003077852,0.0008201303,0.969327,0.0006175054,0.0276076,0.0000204358],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2956243,0.009334292,0.6640179,0.002147882,0.000864501,0.003203398,0.006810425,0.005413274,0.01258404],"genre_scores_gemma":[0.4810103,0.009293726,0.4086466,0.001193095,0.0002166874,0.00424149,0.04937684,0.002108813,0.04391252],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004567625,"threshold_uncertainty_score":0.01528025,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04219067459534841,"score_gpt":0.3797231265398754,"score_spread":0.337532451944527,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}