{"id":"W1490397540","doi":"10.2144/01312st02","title":"Fluorescence Produced by Transfection Reagents Can Be Confused with Green Fluorescent Proteins in Mammalian Cells","year":2001,"lang":"en","type":"article","venue":"BioTechniques","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"Northeast Cancer Centre","funders":"Medical Research Council; Canadian Institutes of Health Research","keywords":"Green fluorescent protein; Aequorea victoria; Transfection; Fluorescence; Fluorescence microscope; Fusion protein; Molecular biology; Biology; Flow cytometry; Reporter gene; Chemistry; Gene expression; Cell biology; Recombinant DNA; Gene; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001330318,0.00019857,0.0001456813,0.00007126958,0.00004676836,0.0000247433,0.0002032823,0.000229688,0.00001290924],"category_scores_gemma":[0.000007365571,0.0001725393,0.00004642731,0.0001421886,0.00007389573,0.000007195135,0.00002958782,0.0001344792,0.000002438625],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000035457,"about_ca_system_score_gemma":0.00004736114,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002285179,"about_ca_topic_score_gemma":0.001248674,"domain_scores_codex":[0.9988387,0.00006632811,0.0002000847,0.0004691686,0.0001429134,0.0002828468],"domain_scores_gemma":[0.9994572,0.000001838542,0.00005374087,0.0003479515,0.00006637554,0.00007285477],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002702926,0.0001205448,0.001554082,0.0000189894,0.00001323675,0.00001544057,0.00005652477,8.131415e-7,0.9904076,0.00000258519,0.003034353,0.004505487],"study_design_scores_gemma":[0.0003524635,0.001004987,0.0008461339,0.00006164255,0.000007818688,0.00001142124,0.0000236604,0.00001058989,0.9828261,0.000005685185,0.01461036,0.0002391733],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965438,0.0001070678,0.001056943,0.001182967,0.00003936535,0.0008681816,0.00004113336,0.00006212763,0.00009841596],"genre_scores_gemma":[0.9974431,0.0005795007,0.0008817019,0.0003939517,0.00007251909,0.0001284249,0.0001112624,0.00002665234,0.0003628801],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01157601,"threshold_uncertainty_score":0.7035948,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01191476016996263,"score_gpt":0.2338063188681291,"score_spread":0.2218915586981664,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}