{"id":"W1490397540","doi":"10.2144/01312st02","title":"Fluorescence Produced by Transfection Reagents Can Be Confused with Green Fluorescent Proteins in Mammalian Cells","year":2001,"lang":"en","type":"article","venue":"BioTechniques","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"Northeast Cancer Centre","funders":"Medical Research Council; Canadian Institutes of Health Research","keywords":"Green fluorescent protein; Aequorea victoria; Transfection; Fluorescence; Fluorescence microscope; Fusion protein; Molecular biology; Biology; Flow cytometry; Reporter gene; Chemistry; Gene expression; Cell biology; Recombinant DNA; Gene; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002589745,0.0009427135,0.001072088,0.0008546578,0.0005861614,0.001111591,0.0007658445,0.001497527,0.002253141],"category_scores_gemma":[0.001835725,0.000771183,0.0006922259,0.001000237,0.0009602284,0.001047937,0.0009022205,0.001933536,0.003113374],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000648524,"about_ca_system_score_gemma":0.0005351734,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003940505,"about_ca_topic_score_gemma":0.0004894354,"domain_scores_codex":[0.997801,0.0007110506,0.0003042257,0.0004294991,0.0005679459,0.0001861879],"domain_scores_gemma":[0.9987673,0.0004931974,0.000239349,0.0002763545,0.000168872,0.00005506224],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000608736,0.00001793196,0.0001229682,0.0001627939,0.000009320049,0.0001967974,0.00007977903,0.00005822159,0.9933625,0.001492529,0.0003854541,0.004050719],"study_design_scores_gemma":[0.000009144021,0.00006417747,0.0002936159,0.00001882549,0.00001591823,0.0004424294,0.00001358127,0.0003118324,0.9803917,0.0003560955,0.01807255,0.00001014656],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.270762,0.009566308,0.6862199,0.00224949,0.001588309,0.0008106464,0.001245454,0.005157076,0.02240096],"genre_scores_gemma":[0.4978381,0.01281086,0.4471204,0.001567989,0.000247967,0.002149949,0.004535626,0.001665092,0.03206404],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.002589745,"threshold_uncertainty_score":0.01369607,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01191476016996263,"score_gpt":0.2338063188681291,"score_spread":0.2218915586981664,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}