{"id":"W1493400306","doi":"10.2144/02324st07","title":"Acrylamide Capture of DNA-Bound Complexes: Electrophoretic Purification of Transcription Factors","year":2002,"lang":"en","type":"article","venue":"BioTechniques","topic":"Genomics and Chromatin Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"Vancouver General Hospital","funders":"","keywords":"DNA; Electrophoretic mobility shift assay; Gel electrophoresis; Oligonucleotide; DNA-binding protein; Molecular biology; Recombinant DNA; Primer (cosmetics); Biology; Transcription (linguistics); Transcription factor; Chemistry; Electrophoresis; DNA ligase; Polyacrylamide gel electrophoresis; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006402987,0.0001107444,0.0001423659,0.00004662138,0.0000262478,0.000006832747,0.0001527209,0.0002126917,0.00002412662],"category_scores_gemma":[0.00001373275,0.0001045209,0.00008904471,0.00007022033,0.0001034811,0.000002399968,0.00001600245,0.00005855986,5.842704e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001494632,"about_ca_system_score_gemma":0.00001212124,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004245111,"about_ca_topic_score_gemma":0.00001538857,"domain_scores_codex":[0.99936,0.0000270746,0.0002421063,0.0001690274,0.00008517713,0.0001166606],"domain_scores_gemma":[0.9994876,0.000003835037,0.0001435244,0.0002694198,0.00007024557,0.00002531282],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000009725538,0.00005788066,0.0002062278,0.00004287255,0.0000165539,1.117856e-7,0.0001200351,0.000003377281,0.9983029,0.0005824094,0.0003031191,0.0003547629],"study_design_scores_gemma":[0.00008929292,0.0002455692,0.001486842,0.00001440181,0.00001479097,0.00000264571,0.00005421126,0.000212442,0.9958434,0.000310602,0.001621526,0.0001042535],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9920102,0.0008081157,0.006534629,0.00005241787,0.0000251341,0.0001696277,0.00004870916,0.00001895551,0.0003322187],"genre_scores_gemma":[0.9958699,0.000995907,0.002862792,0.00001660758,0.0000208612,0.000006633732,0.0001281206,0.00001561217,0.0000835851],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003859689,"threshold_uncertainty_score":0.4262238,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.012262773585971,"score_gpt":0.22184256649783,"score_spread":0.209579792911859,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}