{"id":"W1493400306","doi":"10.2144/02324st07","title":"Acrylamide Capture of DNA-Bound Complexes: Electrophoretic Purification of Transcription Factors","year":2002,"lang":"en","type":"article","venue":"BioTechniques","topic":"Genomics and Chromatin Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"Vancouver General Hospital","funders":"","keywords":"DNA; Electrophoretic mobility shift assay; Gel electrophoresis; Oligonucleotide; DNA-binding protein; Molecular biology; Recombinant DNA; Primer (cosmetics); Biology; Transcription (linguistics); Transcription factor; Chemistry; Electrophoresis; DNA ligase; Polyacrylamide gel electrophoresis; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005226182,0.0009041232,0.0004279357,0.0006692858,0.0003983186,0.0004698103,0.0008868293,0.0003999138,0.001457855],"category_scores_gemma":[0.0005739333,0.0003806395,0.0003556898,0.0004178231,0.0003512577,0.0004059097,0.000356414,0.001330717,0.001312337],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004476848,"about_ca_system_score_gemma":0.0004059459,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001528061,"about_ca_topic_score_gemma":0.002540607,"domain_scores_codex":[0.999384,0.00008787736,0.00004130463,0.0001539263,0.0002363488,0.00009641846],"domain_scores_gemma":[0.9996119,0.000111242,0.00006234463,0.00005676387,0.0001048884,0.00005294841],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000123623,0.00001426864,0.0000500193,0.0000429457,0.000003726232,0.00001755819,0.00001719033,0.00003597897,0.9974831,0.000094669,0.00005584113,0.002172381],"study_design_scores_gemma":[0.00001216005,0.00007459485,0.0007604985,0.000006691663,0.000008433005,0.000239745,0.00001280065,0.0008070613,0.9924488,0.00009794989,0.005521107,0.00001014231],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4240287,0.01305962,0.5531188,0.0006515346,0.0002412145,0.0005637674,0.0009446065,0.001586195,0.00580547],"genre_scores_gemma":[0.5947356,0.007707738,0.3734489,0.0004423163,0.0001313009,0.0004596353,0.00404417,0.0004121544,0.01861824],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001528061,"threshold_uncertainty_score":0.004876971,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.012262773585971,"score_gpt":0.22184256649783,"score_spread":0.209579792911859,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}