{"id":"W1512730941","doi":"10.1111/j.1742-4658.2008.06830.x","title":"Accessory active site residues of <i>Streptomyces</i> sp. N174 chitosanase","year":2009,"lang":"en","type":"article","venue":"FEBS Journal","topic":"Studies on Chitinases and Chitosanases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chitosanase; Glycoside hydrolase; Saturated mutagenesis; Glycosidic bond; Active site; Hydrolase; Residue (chemistry); Serine; Stereochemistry; Lysozyme; Mutant; Chemistry; Site-directed mutagenesis; Mutagenesis; Biochemistry; Biology; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005766193,0.0003513098,0.0001831988,0.0001247942,0.000104323,0.0002288557,0.0001281071,0.0001657087,0.0008068837],"category_scores_gemma":[0.0001523487,0.00008226792,0.0002498449,0.0001619388,0.0001385477,0.0001129109,0.000146417,0.000243788,0.0003115408],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000300394,"about_ca_system_score_gemma":0.0002259903,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008410629,"about_ca_topic_score_gemma":0.0006762078,"domain_scores_codex":[0.9999343,0.000009686708,0.0000066542,0.00001508572,0.00002102881,0.00001324909],"domain_scores_gemma":[0.9998717,0.00001376837,0.00004297696,0.000009123868,0.00001056697,0.00005171217],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001810347,0.00001624211,0.001674224,0.00003614154,0.000006705951,0.0002709284,0.0000205507,0.0001415431,0.995666,0.00006454838,0.00002721449,0.001894868],"study_design_scores_gemma":[0.00003053424,0.0003474716,0.06126318,0.00001487567,0.0000394397,0.002122563,0.0001094776,0.001645982,0.9298313,0.00009430383,0.004477425,0.00002347392],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9979518,0.0003269652,0.0008093482,0.00001908664,0.000007995305,0.000007792431,0.0001408336,0.00002500081,0.0007111374],"genre_scores_gemma":[0.996774,0.0001778588,0.001689785,0.00002101235,0.000004247172,0.00000480073,0.0006642475,0.00001334102,0.0006506378],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008410629,"threshold_uncertainty_score":0.002699316,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009165268747662764,"score_gpt":0.265835439386102,"score_spread":0.2566701706384392,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}