{"id":"W1526218881","doi":"10.1371/journal.pone.0133651","title":"Vpx-Independent Lentiviral Transduction and shRNA-Mediated Protein Knock-Down in Monocyte-Derived Dendritic Cells","year":2015,"lang":"en","type":"article","venue":"PLoS ONE","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Faculté de Médecine, Université Laval; Vlaamse regering; Universiteit Gent; Fonds Wetenschappelijk Onderzoek; York University; Université Laval","keywords":"SAMHD1; Transduction (biophysics); Small hairpin RNA; Biology; Multiplicity of infection; Gene silencing; Cell biology; Viral vector; RNA interference; Immune system; Dendritic cell; Virology; Virus; Cell culture; Gene; Gene knockdown; Immunology; Genetics; Reverse transcriptase; RNA; Recombinant DNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004372375,0.0004550801,0.0004330439,0.0003244519,0.0003573392,0.0005332253,0.0005064894,0.0005143257,0.001390289],"category_scores_gemma":[0.0001731262,0.000280273,0.0003624748,0.000350945,0.0004502602,0.0002390257,0.0003634347,0.001105557,0.0007495961],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003923807,"about_ca_system_score_gemma":0.0004609285,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005228322,"about_ca_topic_score_gemma":0.0008154654,"domain_scores_codex":[0.9993539,0.0001046251,0.0001010168,0.000119815,0.0002114422,0.0001090766],"domain_scores_gemma":[0.9998418,0.00002179353,0.00003640654,0.00004199176,0.00002872593,0.00002933178],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006916166,0.00003992149,0.00009288001,0.00007430245,0.000005418943,0.00005932598,0.00003740101,0.00009252629,0.9969896,0.0005845062,0.0001445776,0.001810242],"study_design_scores_gemma":[0.00001597536,0.0001054289,0.0004547624,0.000004884307,0.00001014941,0.000127444,0.000008971945,0.0006479535,0.9934067,0.00004912375,0.005163498,0.000005145098],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8619198,0.003575398,0.1189623,0.0004423522,0.0004635046,0.001706206,0.005544098,0.001283728,0.006102714],"genre_scores_gemma":[0.9063531,0.002563102,0.07007605,0.0002123139,0.00005826194,0.001427023,0.003266825,0.0001885852,0.01585487],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001390289,"threshold_uncertainty_score":0.00465095,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03595469770136704,"score_gpt":0.220970600200711,"score_spread":0.1850159024993439,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}