{"id":"W1546455379","doi":"10.1111/tra.12073","title":"A Novel Assay for Measurement of Membrane‐Protein Surface Expression using a β‐lactamase Reporter","year":2013,"lang":"en","type":"article","venue":"Traffic","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Biology; Cell biology; Protein expression; Membrane; Reporter gene; Membrane protein; Surface (topology); Molecular biology; Computational biology; Biochemistry; Gene expression; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001772117,0.002272734,0.001686465,0.001491786,0.0005952014,0.001373178,0.002377532,0.002552952,0.001479595],"category_scores_gemma":[0.001390682,0.0008350966,0.001107394,0.001154713,0.001094884,0.001437425,0.001119726,0.004400385,0.001508789],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009675324,"about_ca_system_score_gemma":0.0008340942,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003858693,"about_ca_topic_score_gemma":0.0005494352,"domain_scores_codex":[0.9970431,0.0007752399,0.0002258467,0.0007162269,0.001048584,0.0001910582],"domain_scores_gemma":[0.9981737,0.0006181788,0.000456244,0.0002277927,0.0003250244,0.0001991207],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004860692,0.00006606368,0.0001053542,0.0001346436,0.00001470748,0.00003367513,0.00003272318,0.00004575486,0.9960044,0.0003936676,0.0001734204,0.002946906],"study_design_scores_gemma":[0.0000187135,0.0004017381,0.0007996951,0.00002014032,0.00003022524,0.0005740096,0.00002878026,0.002367072,0.9911885,0.0001966538,0.004342625,0.00003187271],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1080906,0.005396383,0.8772668,0.000580515,0.0006496852,0.0006811733,0.001246912,0.002640953,0.003447078],"genre_scores_gemma":[0.2832642,0.007284406,0.6918088,0.0005368359,0.0003019538,0.002234158,0.00249004,0.0002480295,0.01183169],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.002552952,"threshold_uncertainty_score":0.009371996,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03529654382583925,"score_gpt":0.2503428940485294,"score_spread":0.2150463502226902,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}