{"id":"W1547589600","doi":"10.1002/bab.1079","title":"Functional expression of amyloidogenic human stefins <scp>A</scp> and <scp>B</scp> in <i>Pichia pastoris</i> using codon optimization","year":2013,"lang":"en","type":"article","venue":"Biotechnology and Applied Biochemistry","topic":"Biofuel production and bioconversion","field":"Engineering","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; University of British Columbia","keywords":"Pichia pastoris; Recombinant DNA; Pichia; Molecular biology; Gene; Biochemistry; Expression vector; Biology; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002346178,0.0005237942,0.0003136494,0.00020796,0.0002280798,0.0003288322,0.000181333,0.0002252307,0.0007048109],"category_scores_gemma":[0.0001354984,0.0001915445,0.0002996043,0.0004382528,0.0002260862,0.0001624177,0.0002389806,0.0004777899,0.0003945837],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004217305,"about_ca_system_score_gemma":0.0002756896,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001399433,"about_ca_topic_score_gemma":0.001504903,"domain_scores_codex":[0.9998683,0.00002297103,0.00001666323,0.00002603662,0.00003958711,0.00002645938],"domain_scores_gemma":[0.9999247,0.00001724385,0.00001374252,0.00001252977,0.00001399387,0.00001794066],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006109998,0.00001558676,0.0001081522,0.00002601116,0.000005282795,0.00004650866,0.00001808165,0.00009951508,0.9986078,0.0000931523,0.00006016482,0.0008587254],"study_design_scores_gemma":[0.00001500613,0.0000755232,0.001608416,0.000004647175,0.00001430647,0.0001581443,0.00001818012,0.001485691,0.9936953,0.00005191534,0.002867501,0.000005310263],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9819854,0.0006959384,0.01299458,0.0001439854,0.0000411787,0.00009886248,0.001949688,0.0001845276,0.001905814],"genre_scores_gemma":[0.9729512,0.0008871776,0.01714923,0.00007097979,0.00001515745,0.00009459131,0.004297658,0.00008006735,0.004453887],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001399433,"threshold_uncertainty_score":0.003059924,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008617643945036823,"score_gpt":0.1818890891992275,"score_spread":0.1732714452541906,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}