{"id":"W154997221","doi":"10.2144/02336st03","title":"Purification of Histidine-Tagged T4 RNA Ligase from <i>E. coli</i>","year":2002,"lang":"en","type":"article","venue":"BioTechniques","topic":"RNA modifications and cancer","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Simon Fraser University; Michael Smith Health Research BC","keywords":"RNA ligase; DNA ligase; RNA; Biology; Escherichia coli; Histidine; Genetics; Biochemistry; Computational biology; Molecular biology; Chemistry; DNA; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006756723,0.00009022267,0.00009565536,0.00003271319,0.00004039317,0.000009927135,0.0001583982,0.0001661674,0.00009067383],"category_scores_gemma":[0.00002275641,0.00008812908,0.00006044516,0.00007517287,0.00006352065,0.000003458213,0.00002615566,0.00005030336,0.00001003497],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001496765,"about_ca_system_score_gemma":0.00001516331,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000971347,"about_ca_topic_score_gemma":0.000003966079,"domain_scores_codex":[0.9993885,0.00002684962,0.0001885649,0.0002196413,0.00008170216,0.00009474935],"domain_scores_gemma":[0.9993469,0.000004828572,0.0001082523,0.0004237864,0.00007714734,0.0000390542],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001014504,0.00008427736,0.00009839825,0.000004895784,0.000009038761,3.225489e-7,0.00001683502,6.474475e-7,0.9721212,0.0003890444,0.01888112,0.008384096],"study_design_scores_gemma":[0.00009534792,0.00008082263,0.0004907492,0.00001173839,0.000009561916,7.889818e-7,0.000007080271,0.00003484114,0.8702164,0.000155986,0.1288046,0.00009211713],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9658019,0.007814773,0.01901278,0.0009429035,0.0001039674,0.0003648314,0.0001573309,0.00009916702,0.005702335],"genre_scores_gemma":[0.9895475,0.003606427,0.005557982,0.000165515,0.000131229,0.00005567042,0.0001096879,0.00001407763,0.0008119186],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1099235,"threshold_uncertainty_score":0.3593799,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01912906867185628,"score_gpt":0.2424315051692364,"score_spread":0.2233024364973801,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}