{"id":"W1563923385","doi":"","title":"High-Efficiency Thermal Asymmetric InterLaced (HE-TAIL) PCR for Amplification of Ds Transposon Insertion Sites in Barley","year":2010,"lang":"en","type":"article","venue":"","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Transposable element; Biology; Genome; Genetics; genomic DNA; Multiple displacement amplification; Polymerase chain reaction; DNA; Molecular biology; Computational biology; Gene; DNA extraction","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00102884,0.0006315754,0.0006868106,0.0006183682,0.0002868522,0.0004872518,0.0005928989,0.0005324447,0.001451475],"category_scores_gemma":[0.0009941942,0.0005174754,0.0006596574,0.0003864653,0.0005476784,0.0006473698,0.0005274186,0.001169868,0.001203939],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003851857,"about_ca_system_score_gemma":0.000469099,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004571531,"about_ca_topic_score_gemma":0.001085411,"domain_scores_codex":[0.9991176,0.0001941241,0.0001049419,0.0002701252,0.0002145043,0.00009870838],"domain_scores_gemma":[0.9993976,0.0001989261,0.0001433729,0.0001186266,0.00007904557,0.00006247369],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004499656,0.00001527387,0.0001316831,0.0000532804,0.000004561569,0.00004300363,0.00002461112,0.00009543795,0.9940877,0.0001406715,0.00005074446,0.005308089],"study_design_scores_gemma":[0.00001593587,0.0001390485,0.001961184,0.000009320539,0.00002264154,0.000360808,0.00002802808,0.001541426,0.9928144,0.0001196763,0.002966575,0.00002103724],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3585807,0.001464403,0.6342139,0.0002380204,0.0002152346,0.0004655221,0.0009387206,0.001244166,0.002639359],"genre_scores_gemma":[0.4920195,0.00151952,0.4957275,0.000225632,0.00004850439,0.0003071309,0.002216045,0.0003887334,0.007547362],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001451475,"threshold_uncertainty_score":0.00544107,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007844531036369583,"score_gpt":0.2736301603358195,"score_spread":0.2657856292994499,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}