{"id":"W1587089803","doi":"10.1016/s0580-9517(06)36016-3","title":"16 High-Throughput Strain Construction and Systematic Synthetic Lethal Screening in","year":2007,"lang":"en","type":"book-chapter","venue":"Methods in microbiology","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":136,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"","keywords":"Biology; Gene; Mutant; Saccharomyces cerevisiae; Synthetic lethality; Genetics; Phenotype; Genetic screen; Lethal allele; Computational biology; Synthetic biology; Robustness (evolution); Genome; Model organism; Genetic analysis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009022832,0.001315021,0.001389885,0.001246273,0.0005477797,0.001305188,0.001991742,0.000683924,0.008727062],"category_scores_gemma":[0.0004135539,0.0009976166,0.001463573,0.00164634,0.0005023642,0.0007375428,0.001377964,0.002077307,0.0260914],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005788927,"about_ca_system_score_gemma":0.0006618059,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008816528,"about_ca_topic_score_gemma":0.002215666,"domain_scores_codex":[0.9990539,0.0001137461,0.0001142511,0.0001998021,0.0004199211,0.00009844582],"domain_scores_gemma":[0.9997677,0.00004405898,0.00002366547,0.00009291668,0.00004101473,0.00003061292],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001270907,0.00009563721,0.000182481,0.0003238996,0.00002935377,0.0001346806,0.00008277722,0.0005043292,0.9397644,0.002493425,0.009610836,0.0466511],"study_design_scores_gemma":[0.00002633231,0.0001157384,0.0008063617,0.0000506668,0.00005073866,0.0003349574,0.00002041663,0.001847957,0.8999199,0.0009166802,0.09587397,0.00003637184],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07585201,0.005558084,0.8051301,0.001129248,0.00120527,0.002123892,0.03728339,0.03038015,0.04133775],"genre_scores_gemma":[0.124954,0.008556059,0.6266461,0.0005985191,0.000138703,0.002611838,0.0996006,0.006501922,0.1303923],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.008727062,"threshold_uncertainty_score":0.02919495,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04079028095448186,"score_gpt":0.3573077640415299,"score_spread":0.316517483087048,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}