{"id":"W159816686","doi":"10.2144/02321st04","title":"Live-Cell Nucleocytoplasmic Protein Shuttle Assay Utilizing Laser Confocal Microscopy and FRAP","year":2002,"lang":"en","type":"article","venue":"BioTechniques","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Canadian Institutes of Health Research; Oklahoma State University; University of Chicago; Oklahoma Agricultural Experiment Station; McMaster University; Hereditary Disease Foundation","keywords":"Fluorescence recovery after photobleaching; Green fluorescent protein; Cell biology; Protein subcellular localization prediction; Fluorescence microscope; Fusion protein; Confocal microscopy; Nucleus; Nuclear transport; Biology; Subcellular localization; Cellular compartment; Live cell imaging; Photobleaching; Nuclear protein; Nuclear pore; Compartment (ship); Confocal; Nuclear localization sequence; Cell nucleus; Fluorescence; Cytoplasm; Cell; Biochemistry; Recombinant DNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001062885,0.00112804,0.001326606,0.001224678,0.0009099243,0.0008255367,0.001616882,0.001041556,0.002827078],"category_scores_gemma":[0.0005873856,0.0004027134,0.0006484221,0.0007248648,0.0009226144,0.001354015,0.0009315476,0.002117058,0.002348675],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001027983,"about_ca_system_score_gemma":0.0007036535,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001377216,"about_ca_topic_score_gemma":0.002050268,"domain_scores_codex":[0.9991472,0.0001434222,0.0000828393,0.0002089204,0.0003255602,0.0000921593],"domain_scores_gemma":[0.9994332,0.0001792915,0.0000884797,0.0001036801,0.00009971357,0.00009559329],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006361386,0.00006472356,0.00008731131,0.0001139961,0.00001398316,0.00004856205,0.00003019016,0.0001171935,0.9970182,0.000590715,0.0002180289,0.001633513],"study_design_scores_gemma":[0.00001591716,0.0001160506,0.0004775587,0.000006809186,0.00001808311,0.0001968456,0.00001233103,0.002379957,0.9927006,0.0002258564,0.003830195,0.00001971503],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3193625,0.004250587,0.6582811,0.0006105846,0.0003471807,0.001111433,0.00302243,0.003595628,0.00941866],"genre_scores_gemma":[0.4202792,0.006492305,0.5480423,0.0002652332,0.0001077785,0.001299312,0.007033238,0.0005381286,0.01594247],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002827078,"threshold_uncertainty_score":0.009457529,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0112485939381351,"score_gpt":0.2558248333890938,"score_spread":0.2445762394509587,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}