{"id":"W1653026469","doi":"10.1071/rdv17n2ab46","title":"46 EFFICIENT TRANSFECTION OF PLASMID DNA INTO CELLS FOR USE AS NUCLEAR DONORS","year":2004,"lang":"en","type":"article","venue":"Reproduction Fertility and Development","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Natural Sciences and Engineering Research Council of Canada; Ontario Ministry of Agriculture, Food and Rural Affairs","keywords":"Transfection; Electroporation; Molecular biology; Somatic cell nuclear transfer; Plasmid; Recombinant DNA; Cell culture; Green fluorescent protein; Biology; Fluorescence microscope; Chemistry; Andrology; DNA; Gene; Fluorescence; Genetics; Medicine; Embryogenesis","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001821022,0.00008988225,0.00009037467,0.00003194106,0.00008443428,0.00001084521,0.00003155294,0.00006659269,0.000004060658],"category_scores_gemma":[0.00004122449,0.00009089887,0.00003619326,0.00004456303,0.00003597664,0.000003479117,0.00002047218,0.00003099832,0.000002114723],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002725842,"about_ca_system_score_gemma":0.00005115391,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003063489,"about_ca_topic_score_gemma":0.00001676649,"domain_scores_codex":[0.999265,0.000008812908,0.0001903318,0.0003607893,0.00007070515,0.0001043527],"domain_scores_gemma":[0.9996757,0.00000381258,0.00002722441,0.0001849359,0.00006346512,0.00004489265],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004112533,0.0001275704,0.0002204161,0.0001222387,0.00003390576,1.409619e-7,0.0008297072,0.01144278,0.9801665,0.00001907248,0.00002959386,0.006596806],"study_design_scores_gemma":[0.0004614904,0.0001394795,0.01238881,0.00001127534,0.000007671708,0.000003889467,0.0001005903,0.0000371483,0.969608,0.00003109697,0.01710687,0.0001036638],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9654578,0.0001510098,0.03380999,0.00007139301,0.0002182875,0.0002579756,0.000002138908,0.00001413278,0.0000172958],"genre_scores_gemma":[0.9918054,0.0000592795,0.007997065,0.00001793151,0.0000319106,0.00001426419,0.00001852187,0.000009589075,0.00004606888],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0263476,"threshold_uncertainty_score":0.3706748,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009993008502002214,"score_gpt":0.2557049464989746,"score_spread":0.2457119379969724,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}