{"id":"W1653026469","doi":"10.1071/rdv17n2ab46","title":"46 EFFICIENT TRANSFECTION OF PLASMID DNA INTO CELLS FOR USE AS NUCLEAR DONORS","year":2004,"lang":"en","type":"article","venue":"Reproduction Fertility and Development","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Natural Sciences and Engineering Research Council of Canada; Ontario Ministry of Agriculture, Food and Rural Affairs","keywords":"Transfection; Electroporation; Molecular biology; Somatic cell nuclear transfer; Plasmid; Recombinant DNA; Cell culture; Green fluorescent protein; Biology; Fluorescence microscope; Chemistry; Andrology; DNA; Gene; Fluorescence; Genetics; Medicine; Embryogenesis","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001178778,0.0006589746,0.0008646984,0.0007224938,0.0004369712,0.0008409102,0.0004776581,0.0005897335,0.004698749],"category_scores_gemma":[0.0004941021,0.0003731736,0.0005214732,0.0007608453,0.000462152,0.0004240622,0.0006110965,0.0008552493,0.003513767],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003420752,"about_ca_system_score_gemma":0.0003840752,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003901575,"about_ca_topic_score_gemma":0.0006833874,"domain_scores_codex":[0.9987098,0.0003207669,0.0001686272,0.0002936138,0.0003808079,0.0001264121],"domain_scores_gemma":[0.9997836,0.00004960609,0.00003173103,0.00007046333,0.00004314779,0.00002155998],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005935816,0.00004811433,0.0001110671,0.0001349151,0.00001113143,0.00007360464,0.0000690155,0.0001051154,0.9914114,0.0007639091,0.0003197508,0.006892635],"study_design_scores_gemma":[0.00003029719,0.0002358842,0.0005915397,0.00002244678,0.00003225537,0.0003033447,0.00002205441,0.001085116,0.9625545,0.0001563275,0.03495342,0.00001286642],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.4916891,0.01145172,0.4475715,0.0004106827,0.0007656862,0.003998117,0.006780548,0.002851062,0.03448162],"genre_scores_gemma":[0.6989502,0.01200399,0.2191582,0.000254033,0.00009792277,0.003114133,0.0183072,0.0006256425,0.04748871],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004698749,"threshold_uncertainty_score":0.01571888,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009993008502002214,"score_gpt":0.2557049464989746,"score_spread":0.2457119379969724,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}