{"id":"W1701915267","doi":"10.1007/s00726-015-2113-z","title":"Soluble expression, purification and functional characterization of a coil peptide composed of a positively charged and hydrophobic motif","year":2015,"lang":"en","type":"article","venue":"Amino Acids","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Montréal; Polytechnique Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Peptide; Chemistry; Motif (music); Coiled coil; Proteomics; Biochemistry; Biophysics; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002137775,0.0002329705,0.0002078815,0.000175293,0.0001827907,0.0002505952,0.0001556516,0.0001586063,0.0005056522],"category_scores_gemma":[0.0001694686,0.0001290697,0.0002344866,0.000275563,0.0001840055,0.0001828344,0.0001946289,0.000362074,0.0003653665],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003041496,"about_ca_system_score_gemma":0.0003158278,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006661066,"about_ca_topic_score_gemma":0.0007171876,"domain_scores_codex":[0.999894,0.00001665971,0.00001112342,0.00002300991,0.00002473292,0.00003050187],"domain_scores_gemma":[0.9998372,0.00002877419,0.00003309253,0.0000182105,0.00003915403,0.00004353392],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005854608,0.00001833359,0.0001291796,0.00002600249,0.00000345596,0.00003922793,0.00001473242,0.00008728958,0.9988893,0.0001341688,0.00002843903,0.0005713998],"study_design_scores_gemma":[0.00001023401,0.00007951095,0.001777733,0.000002991471,0.00001206084,0.0001739586,0.00001824737,0.0008885455,0.9946296,0.00006048996,0.0023428,0.0000038204],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9860912,0.0006411368,0.01144339,0.0001093055,0.00002338272,0.00005977187,0.0009444278,0.00004593925,0.0006414313],"genre_scores_gemma":[0.9741192,0.0008195675,0.01625625,0.00006646577,0.00002661153,0.00005009058,0.004329773,0.00005677056,0.004275373],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006661066,"threshold_uncertainty_score":0.002206743,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01359746776559018,"score_gpt":0.2101158630935678,"score_spread":0.1965183953279777,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}