{"id":"W1793459287","doi":"10.1093/bioinformatics/btv420","title":"CEMAsuite: open source degenerate PCR primer design","year":2015,"lang":"en","type":"article","venue":"Bioinformatics","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Division of Chemical, Bioengineering, Environmental, and Transport Systems; Egg Farmers of Canada; Louisiana State University; National Science Foundation","keywords":"Primer (cosmetics); Suite; Computer science; Source code; Computational biology; Sequence (biology); Coding (social sciences); Multiple sequence alignment; Sequence alignment; Biology; Genetics; Programming language; Gene; Peptide sequence; Mathematics; Statistics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.005835146,0.004165983,0.003615377,0.004496634,0.001728724,0.002768286,0.005255073,0.00230037,0.1058047],"category_scores_gemma":[0.0115095,0.003924326,0.002863572,0.002211276,0.001693738,0.001816287,0.002474316,0.005459417,0.08298575],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00106526,"about_ca_system_score_gemma":0.002512324,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000906691,"about_ca_topic_score_gemma":0.002705505,"domain_scores_codex":[0.9937006,0.00189713,0.0006214325,0.001922047,0.001327494,0.0005312779],"domain_scores_gemma":[0.9967552,0.001486383,0.0003751228,0.0005729363,0.0005868224,0.0002234995],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.003643106,0.0005829317,0.002206146,0.005932652,0.0004133567,0.001191503,0.0008892831,0.004512095,0.2161429,0.02336626,0.5039901,0.2371296],"study_design_scores_gemma":[0.0007170558,0.000642242,0.001937626,0.0006417589,0.0002198381,0.001613827,0.0001539629,0.03270714,0.2404599,0.01577348,0.7046244,0.0005087243],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"methods","genre_gemma":"software","genre_scores_codex":[0.00458551,0.001273971,0.8552584,0.0003133153,0.000803622,0.002205153,0.03269234,0.0918114,0.01105627],"genre_scores_gemma":[0.01227456,0.0004840299,0.9070566,0.0005414579,0.000112319,0.006871236,0.03594079,0.02397775,0.01274137],"genre_candidate":"software","genre_consensus":null,"teacher_disagreement_score":0.1058047,"threshold_uncertainty_score":0.3539521,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05379762823424428,"score_gpt":0.2991767766964953,"score_spread":0.245379148462251,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}