{"id":"W1816644442","doi":"10.1186/1471-2091-6-29","title":"A novel mass spectrometry-based assay for GSK-3β activity","year":2005,"lang":"en","type":"article","venue":"BMC Biochemistry","topic":"Wnt/β-catenin signaling in development and cancer","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University; St Joseph's Health Centre; Lawson Health Research Institute","funders":"Schulich School of Medicine and Dentistry; Canadian Institutes of Health Research; Lawson Health Research Institute","keywords":"Phosphorylation; GSK-3; Chemistry; Peptide; Biochemistry; GSK3B; Mass spectrometry; Kinase; Enzyme; Recombinant DNA; Substrate (aquarium); Substrate-level phosphorylation; Glycogen synthase; Molecular biology; Chromatography; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001021748,0.001640214,0.0005921109,0.001491114,0.0003814279,0.0007001454,0.001086811,0.001402708,0.001679159],"category_scores_gemma":[0.000923463,0.0004912021,0.0006444295,0.0007742248,0.0004884038,0.0008100334,0.0005420041,0.001457749,0.001633791],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004831532,"about_ca_system_score_gemma":0.0005242825,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001811944,"about_ca_topic_score_gemma":0.0006896671,"domain_scores_codex":[0.9987373,0.0001829298,0.00007817065,0.000348268,0.0005841997,0.00006909135],"domain_scores_gemma":[0.9992083,0.0002312243,0.0002168118,0.00005847938,0.0001762902,0.0001089981],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006712991,0.00005315799,0.0004351192,0.00009951433,0.0000200887,0.00005142108,0.00001348303,0.00005123223,0.9951416,0.00007598926,0.0001269481,0.003864333],"study_design_scores_gemma":[0.00003004061,0.0004832339,0.003606847,0.00002053546,0.00006176436,0.001178724,0.00002435165,0.00286643,0.9879582,0.0001934132,0.003545279,0.00003122928],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4051305,0.01603647,0.5627539,0.0009956147,0.0009209607,0.000919975,0.003810678,0.002568396,0.006863546],"genre_scores_gemma":[0.3923115,0.008525794,0.5819091,0.001016341,0.0003974583,0.001511296,0.003332568,0.0001664996,0.01082944],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001679159,"threshold_uncertainty_score":0.005617321,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01717549288853162,"score_gpt":0.2607506344854686,"score_spread":0.243575141596937,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}