{"id":"W1844860071","doi":"10.1101/pdb.prot086256","title":"Monitoring Autophagic Flux by Using Lysosomal Inhibitors and Western Blotting of Endogenous MAP1LC3B","year":2015,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Autophagy in Disease and Therapy","field":"Medicine","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University; University of British Columbia; BC Cancer Agency","funders":"Canadian Institutes of Health Research","keywords":"Autophagy; Flux (metallurgy); Endogeny; Lysosome; Blot; Drug; Chemistry; Cell biology; Pharmacology; Biochemistry; Biology; Enzyme; Apoptosis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003298251,0.0002374371,0.0004497622,0.0001072288,0.0000905924,0.00004664478,0.0001249556,0.0001214376,0.00001168384],"category_scores_gemma":[0.00007352463,0.0002325022,0.00008419832,0.0001515687,0.00009141022,0.0001542681,0.0001041997,0.0001933866,0.000008490654],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001226781,"about_ca_system_score_gemma":0.000226197,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005930902,"about_ca_topic_score_gemma":1.878565e-7,"domain_scores_codex":[0.998391,0.00005356407,0.0004504445,0.0003391216,0.0003941644,0.0003716624],"domain_scores_gemma":[0.9988788,0.0000390748,0.0002242663,0.0003712782,0.000128644,0.0003579195],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001654573,0.000211766,0.1814953,0.0003318782,0.00008092974,0.0001041597,0.0001961146,0.000009649024,0.8167539,0.0000617543,0.00001573828,0.0005733436],"study_design_scores_gemma":[0.003419153,0.0004320029,0.01327476,0.001182216,0.00008053608,0.00001840628,0.00005070553,0.0002757564,0.9790833,0.00001259938,0.001940937,0.0002296551],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9750546,0.001142071,0.00008750515,0.00002402138,0.0001067062,0.02330778,0.000006309915,0.0001328615,0.0001381155],"genre_scores_gemma":[0.9906235,0.000005127232,0.001214472,0.00004552018,0.0004023087,0.007560635,5.193507e-7,0.00006175194,0.00008612168],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1682205,"threshold_uncertainty_score":0.9481166,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06894107139366758,"score_gpt":0.3213398820380909,"score_spread":0.2523988106444233,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}