{"id":"W1869269119","doi":"10.1101/pdb.top070391","title":"Techniques for the Detection of Autophagy in Primary Mammalian Cells","year":2015,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Autophagy in Disease and Therapy","field":"Medicine","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University; BC Cancer Agency","funders":"Biotechnology and Biological Sciences Research Council; National Institute for Health and Care Research; Lady Tata Memorial Trust; Natural Sciences and Engineering Research Council of Canada; Wellcome Trust","keywords":"Autophagy; Mitophagy; Cell biology; Cytoplasm; Flow cytometry; Endoplasmic reticulum; Organelle; Programmed cell death; Mitochondrion; Chemistry; Blot; Unfolded protein response; Biology; Apoptosis; Biochemistry; Molecular biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003900623,0.003418249,0.001945548,0.004220533,0.002155094,0.00108629,0.003587515,0.001936504,0.01362777],"category_scores_gemma":[0.001973373,0.002126358,0.001634527,0.00284034,0.001074558,0.001604894,0.001478988,0.006222501,0.01907814],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001054301,"about_ca_system_score_gemma":0.001213577,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009545734,"about_ca_topic_score_gemma":0.002923625,"domain_scores_codex":[0.9967393,0.0006719314,0.000490804,0.000766276,0.001028385,0.0003031882],"domain_scores_gemma":[0.9979562,0.0006660385,0.0002007133,0.0005529818,0.0004845683,0.0001395997],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00005925014,0.00009706995,0.00009097736,0.0005754576,0.00002354269,0.0001256756,0.00008353172,0.00008461846,0.9845954,0.001495006,0.002990348,0.009779016],"study_design_scores_gemma":[0.0001009631,0.0004701505,0.002724194,0.0002916358,0.0001683005,0.001991197,0.00007567342,0.00242041,0.8152807,0.002617363,0.173769,0.00009039768],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.008123843,0.007167397,0.9593412,0.0005925103,0.0009621818,0.002881302,0.003169771,0.006294568,0.01146721],"genre_scores_gemma":[0.01511739,0.01017084,0.9390239,0.0007860422,0.000291272,0.01387822,0.009563187,0.00106298,0.01010623],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01362777,"threshold_uncertainty_score":0.04558945,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03189196629084892,"score_gpt":0.3102703160403585,"score_spread":0.2783783497495096,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}