{"id":"W1870558638","doi":"10.1128/aem.01248-15","title":"Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli","year":2015,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":282,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University; University of Waterloo","funders":"","keywords":"Recombineering; CRISPR; Cas9; Biology; Genome editing; Genetics; Plasmid; Homologous recombination; Escherichia coli; Gene; DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003913096,0.0004449075,0.0003875493,0.0002600657,0.0002448133,0.0006113756,0.000437888,0.0004770233,0.0006141973],"category_scores_gemma":[0.0003960525,0.0002594228,0.0003484353,0.0002184528,0.0003759762,0.0002061155,0.0005010045,0.0009750103,0.0006055378],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003460757,"about_ca_system_score_gemma":0.0004386489,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001059206,"about_ca_topic_score_gemma":0.001359754,"domain_scores_codex":[0.9994797,0.00008266922,0.0000603532,0.000117807,0.0001942557,0.00006525221],"domain_scores_gemma":[0.9997532,0.00005300379,0.00006411783,0.00006189931,0.00003006579,0.00003771084],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002323061,0.00001434709,0.0001056025,0.00002327001,0.000003583165,0.00006053301,0.00001111717,0.0001833074,0.9976992,0.0002316823,0.0000237467,0.001620398],"study_design_scores_gemma":[0.000003795285,0.00007059835,0.000494166,0.000003591138,0.00000771321,0.0001635902,0.00001146698,0.001219591,0.9958444,0.00005598083,0.002117466,0.000007660568],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8079019,0.0007892037,0.1857482,0.0002246323,0.00008585318,0.000218306,0.0006060647,0.001029709,0.003396288],"genre_scores_gemma":[0.8902121,0.0006128366,0.1042751,0.0001012655,0.00000687247,0.00008175839,0.0006922271,0.0001303441,0.003887468],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001059206,"threshold_uncertainty_score":0.002510965,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005467233457581778,"score_gpt":0.1992563040222078,"score_spread":0.193789070564626,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}