{"id":"W1870558638","doi":"10.1128/aem.01248-15","title":"Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli","year":2015,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":282,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University; University of Waterloo","funders":"","keywords":"Recombineering; CRISPR; Cas9; Biology; Genome editing; Genetics; Plasmid; Homologous recombination; Escherichia coli; Gene; DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001677198,0.00019628,0.0001820334,0.0000233609,0.00006419155,0.00001412415,0.0001146507,0.0001280355,0.000004606444],"category_scores_gemma":[0.00000289455,0.0001502388,0.00002180664,0.00004156464,0.0001005394,0.000002220613,0.0001502813,0.0001065832,0.000004402086],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005465393,"about_ca_system_score_gemma":0.000015868,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000248213,"about_ca_topic_score_gemma":0.00002312907,"domain_scores_codex":[0.9990861,0.00001655272,0.0001990282,0.000368109,0.00003917117,0.0002910576],"domain_scores_gemma":[0.9996156,0.00001041354,0.00004981907,0.0002474405,0.000003457143,0.00007330839],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001567226,0.00002952898,0.001673212,0.00001831086,0.00003798195,0.000004451907,0.00008975189,0.00836379,0.9893875,0.00003028929,0.0001298053,0.00007872443],"study_design_scores_gemma":[0.00288434,0.0005693475,0.003434566,0.00002571621,0.00003521354,0.0002216019,0.003001574,0.001512512,0.9751611,0.000005701394,0.01267297,0.0004753977],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9964573,0.0008194937,0.00201969,0.00005049053,0.00008721039,0.0003118051,0.00001743839,0.00001533674,0.0002212476],"genre_scores_gemma":[0.9987763,0.0001247518,0.0005814866,0.00007804886,0.00007575607,0.00006974871,0.0002075961,0.00002736293,0.00005894118],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01422637,"threshold_uncertainty_score":0.6126561,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005467233457581778,"score_gpt":0.1992563040222078,"score_spread":0.193789070564626,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}