{"id":"W1888578572","doi":"10.1007/978-1-4939-3124-8_8","title":"Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy, and Live Cell Imaging","year":2015,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Plant Reproductive Biology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan; University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Microtubule; Immunofluorescence; Live cell imaging; Plant cell; Transmission electron microscopy; Cell biology; Indirect immunofluorescence; Biophysics; Electron tomography; Tubulin; Biology; Cell; Nanotechnology; Materials science; Biochemistry; Antibody; Scanning transmission electron microscopy","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001182887,0.001004187,0.0008214637,0.001694647,0.001810576,0.001364299,0.001591615,0.001220829,0.001697562],"category_scores_gemma":[0.0008029145,0.001050943,0.0005815692,0.001436075,0.001272523,0.002380014,0.001134987,0.003555045,0.001568682],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001367915,"about_ca_system_score_gemma":0.001065122,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002335376,"about_ca_topic_score_gemma":0.00662358,"domain_scores_codex":[0.999529,0.00010574,0.00004144146,0.00008804489,0.0001864273,0.00004925193],"domain_scores_gemma":[0.9994833,0.0001172333,0.00007309307,0.0001554601,0.000118815,0.00005205336],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001067187,0.00009510895,0.000383698,0.0008326414,0.0000282907,0.0001261245,0.0001755702,0.0004669245,0.9334094,0.00994182,0.001921418,0.0525124],"study_design_scores_gemma":[0.00004451216,0.0001214461,0.002867879,0.0001714167,0.00008391363,0.001538292,0.0001235605,0.006354647,0.9049792,0.008752762,0.07484595,0.0001163906],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.05310084,0.03811471,0.897646,0.002387689,0.0004574238,0.0004621503,0.001505241,0.001533093,0.004792801],"genre_scores_gemma":[0.1301938,0.05515645,0.7974536,0.0005132879,0.0001936414,0.001300887,0.00167459,0.0007892831,0.01272442],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002335376,"threshold_uncertainty_score":0.009924948,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0138402148323781,"score_gpt":0.3764553751583934,"score_spread":0.3626151603260153,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}