{"id":"W1910208674","doi":"10.3390/insects6030746","title":"Development of a Real-Time qPCR Assay for Quantification of Covert Baculovirus Infections in a Major African Crop Pest","year":2015,"lang":"en","type":"article","venue":"Insects","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Biotechnology and Biological Sciences Research Council; Directorate for Biological Sciences; Department for International Development; Natural Environment Research Council; Sight Research UK","keywords":"Biology; PEST analysis; Instar; TaqMan; Virology; Host (biology); Wolbachia; Outbreak; Real-time polymerase chain reaction; Larva; Pathogen; Virus; Covert; Baculoviridae; Spodoptera; Ecology; Microbiology; Botany; Genetics; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001961214,0.001303079,0.001301701,0.001324591,0.0004799483,0.0008876887,0.0008353243,0.00115803,0.001889522],"category_scores_gemma":[0.001871775,0.00101045,0.000895791,0.0009055086,0.0007409923,0.0007198769,0.000535982,0.001681219,0.001326265],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005515256,"about_ca_system_score_gemma":0.0008093034,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006113097,"about_ca_topic_score_gemma":0.00123642,"domain_scores_codex":[0.9981369,0.0003032712,0.0001354492,0.0007025827,0.0005710214,0.0001508638],"domain_scores_gemma":[0.9988865,0.0004688158,0.0001971609,0.0001093095,0.0002802213,0.00005801081],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003335437,0.00004698717,0.0005527676,0.00009804573,0.000007393857,0.00001681343,0.00006332186,0.0002330742,0.9955794,0.0001444536,0.00005636439,0.003168055],"study_design_scores_gemma":[0.00002475215,0.0009735189,0.006208557,0.00008050197,0.00006404932,0.0002802612,0.00009833458,0.01252099,0.973255,0.0003107114,0.006133584,0.00004978187],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3321803,0.001836725,0.6509775,0.0002295287,0.0003577335,0.002406573,0.005115036,0.001877313,0.005019346],"genre_scores_gemma":[0.2013996,0.001930438,0.7750587,0.0003237304,0.0001150685,0.00516924,0.007551178,0.0002795926,0.008172498],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001961214,"threshold_uncertainty_score":0.01037204,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02821403591908794,"score_gpt":0.2928015468943805,"score_spread":0.2645875109752926,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}