{"id":"W1919801225","doi":"10.1016/j.febslet.2015.09.028","title":"Functional roles in<i>S</i>‐adenosyl‐<scp>l</scp>‐methionine binding and catalysis for active site residues of the thiostrepton resistance methyltransferase","year":2015,"lang":"en","type":"article","venue":"FEBS Letters","topic":"Cancer-related gene regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"National Institute of General Medical Sciences; National Institute of Allergy and Infectious Diseases; Natural Sciences and Engineering Research Council of Canada; Howard Hughes Medical Institute; National Institute of Food and Agriculture; National Institutes of Health; National Science Foundation","keywords":"Thiostrepton; Active site; Binding site; Asparagine; Biochemistry; Amino acid; Chemistry; Methionine; Mutant; Arginine; Methyltransferase; Enzyme; Streptomyces; Stereochemistry; Ribosome; Biology; Methylation; Bacteria; Genetics; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001586407,0.0002347584,0.0001260346,0.00009515732,0.0001559162,0.0003110257,0.0001857581,0.0004193732,0.000677964],"category_scores_gemma":[0.0002120464,0.0001245236,0.0001567746,0.00008359889,0.0004175034,0.0002032314,0.0001054957,0.0002997735,0.0002243235],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003027949,"about_ca_system_score_gemma":0.0001846143,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008360632,"about_ca_topic_score_gemma":0.0007006848,"domain_scores_codex":[0.999898,0.00003020828,0.000008104676,0.00001836751,0.00002358246,0.00002170513],"domain_scores_gemma":[0.9999022,0.00003016733,0.00002274259,0.000009530287,0.000009325051,0.00002594289],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001988355,0.00001513084,0.0004738776,0.00001533156,0.000002640534,0.00007687976,0.00002988992,0.0001456064,0.9979829,0.0002955893,0.00000923931,0.000754228],"study_design_scores_gemma":[0.00001431033,0.0001230025,0.009534404,0.000002856792,0.000009097083,0.0003087962,0.00007672689,0.001773203,0.9873385,0.0001662265,0.0006454636,0.000007331927],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998552,0.0003367507,0.0005738481,0.00005444177,0.000003386098,0.000002792214,0.00002790195,0.00001194704,0.0004369849],"genre_scores_gemma":[0.9992724,0.00007938528,0.0003450813,0.000009326841,0.000001171809,0.000001963525,0.00003929601,0.000003453074,0.00024787],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008360632,"threshold_uncertainty_score":0.002268016,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01146055250325213,"score_gpt":0.2276309994558524,"score_spread":0.2161704469526003,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}