{"id":"W1919801225","doi":"10.1016/j.febslet.2015.09.028","title":"Functional roles in<i>S</i>‐adenosyl‐<scp>l</scp>‐methionine binding and catalysis for active site residues of the thiostrepton resistance methyltransferase","year":2015,"lang":"en","type":"article","venue":"FEBS Letters","topic":"Cancer-related gene regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"National Institute of General Medical Sciences; National Institute of Allergy and Infectious Diseases; Natural Sciences and Engineering Research Council of Canada; Howard Hughes Medical Institute; National Institute of Food and Agriculture; National Institutes of Health; National Science Foundation","keywords":"Thiostrepton; Active site; Binding site; Asparagine; Biochemistry; Amino acid; Chemistry; Methionine; Mutant; Arginine; Methyltransferase; Enzyme; Streptomyces; Stereochemistry; Ribosome; Biology; Methylation; Bacteria; Genetics; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002477608,0.0001254806,0.0001442627,0.00007830931,0.00006583698,0.00001387213,0.00009258758,0.0001194412,0.000001011832],"category_scores_gemma":[0.0002504084,0.0001075247,0.00008647508,0.0001932462,0.0001006089,0.00001136666,0.00004189015,0.00009442919,6.957493e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005149661,"about_ca_system_score_gemma":0.00006259089,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004211629,"about_ca_topic_score_gemma":0.0009279367,"domain_scores_codex":[0.999095,0.00008210521,0.0001964362,0.0002951073,0.0001680002,0.0001633084],"domain_scores_gemma":[0.9994501,0.00008433444,0.0001094595,0.0002344985,0.00007558467,0.00004597328],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001652921,0.00001543338,0.00461589,0.00002147301,0.00007479895,3.913106e-7,0.0004478466,0.001586757,0.9837649,0.00001581898,0.009068775,0.0002226317],"study_design_scores_gemma":[0.00089828,0.00005332745,0.02278969,0.00004036441,0.00006328634,0.000002560928,0.0002604744,0.00002672151,0.9692098,0.00005593134,0.006529257,0.00007026617],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9950082,0.000969311,0.00112132,0.002353028,0.0001421215,0.0002457342,0.00008063666,0.00000657531,0.00007308022],"genre_scores_gemma":[0.9982532,0.00006269662,0.0004668766,0.0003082031,0.000150928,0.00003566668,0.0002265372,0.00001872812,0.0004771797],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0181738,"threshold_uncertainty_score":0.438473,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01146055250325213,"score_gpt":0.2276309994558524,"score_spread":0.2161704469526003,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}