{"id":"W1927158622","doi":"10.1002/biot.201400662","title":"Low glucose depletes glycan precursors, reduces site occupancy and galactosylation of a monoclonal antibody in CHO cell culture","year":2015,"lang":"en","type":"article","venue":"Biotechnology Journal","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":55,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada","keywords":"Nucleotide sugar; Glycosylation; Intracellular; Chemistry; Biochemistry; Monoclonal antibody; Cell culture; Energy charge; Nucleotide; Chinese hamster ovary cell; Glycation; Glycoprotein; Carbohydrate metabolism; Adenylate kinase; Antibody; Biology; Enzyme; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002328595,0.0003360746,0.0004349569,0.0001576055,0.0001121673,0.0004776657,0.0003584855,0.0002273911,0.0005048708],"category_scores_gemma":[0.0001593263,0.0001357153,0.0001962128,0.0002430893,0.0001759706,0.0001782533,0.0002994913,0.0004948312,0.0003079244],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003582834,"about_ca_system_score_gemma":0.000201398,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001055093,"about_ca_topic_score_gemma":0.001572303,"domain_scores_codex":[0.9997353,0.00005529292,0.00003668383,0.00004850908,0.00008922127,0.00003494952],"domain_scores_gemma":[0.9998612,0.00003067435,0.00003119933,0.00002718286,0.00002643505,0.0000232148],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003544299,0.00001066137,0.00008327099,0.00001619468,0.000001888306,0.00001327808,0.00000563599,0.00001917693,0.9994342,0.00001441919,0.00001139455,0.0003544597],"study_design_scores_gemma":[0.000004954813,0.0001922585,0.00159586,0.00000419163,0.00001014556,0.00006321345,0.000009492564,0.0002995718,0.9966351,0.00001393687,0.001168168,0.000003092837],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9887813,0.001856397,0.006827761,0.0001019149,0.00004173034,0.00006128683,0.0006553098,0.0001043839,0.001569964],"genre_scores_gemma":[0.9752477,0.001909895,0.01566571,0.0001183642,0.00002213918,0.0001530671,0.002408158,0.00008391555,0.004390931],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001055093,"threshold_uncertainty_score":0.002599537,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008056701789126269,"score_gpt":0.2635344966695335,"score_spread":0.2554777948804072,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}