{"id":"W1927158622","doi":"10.1002/biot.201400662","title":"Low glucose depletes glycan precursors, reduces site occupancy and galactosylation of a monoclonal antibody in CHO cell culture","year":2015,"lang":"en","type":"article","venue":"Biotechnology Journal","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":55,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada","keywords":"Nucleotide sugar; Glycosylation; Intracellular; Chemistry; Biochemistry; Monoclonal antibody; Cell culture; Energy charge; Nucleotide; Chinese hamster ovary cell; Glycation; Glycoprotein; Carbohydrate metabolism; Adenylate kinase; Antibody; Biology; Enzyme; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001532115,0.0001460148,0.0001686355,0.0001415767,0.00006390724,0.00002351831,0.0001386095,0.0004276288,0.000007819553],"category_scores_gemma":[0.0001123414,0.000123172,0.00006280755,0.0001361426,0.0001345793,0.00001251514,0.00009865328,0.0002575569,0.000001931081],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001829033,"about_ca_system_score_gemma":0.0001091892,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001973212,"about_ca_topic_score_gemma":0.00002080655,"domain_scores_codex":[0.9991152,0.00006365335,0.000263229,0.0002365346,0.000107003,0.0002143956],"domain_scores_gemma":[0.9994031,0.00000501506,0.0002145701,0.0001633801,0.0000950608,0.0001188718],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001914909,0.000137485,0.04958268,0.00001372693,0.00001854087,0.000008922184,0.00009916187,0.00003717096,0.9460458,0.00001475278,0.001654072,0.002196257],"study_design_scores_gemma":[0.00115735,0.0007382386,0.007797636,0.00005470878,0.00001416519,0.0001668566,0.0001590671,0.00008337852,0.9859853,0.0004347017,0.003250768,0.0001577921],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9919013,0.007106707,0.0003703723,0.0001652215,0.0001745045,0.0001296198,0.00002161767,0.00001383526,0.0001167808],"genre_scores_gemma":[0.9966016,0.002403272,0.0007063167,0.00004216435,0.0001380097,0.00000631417,0.00004006338,0.00001219007,0.00005007209],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04178504,"threshold_uncertainty_score":0.5022807,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008056701789126269,"score_gpt":0.2635344966695335,"score_spread":0.2554777948804072,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}