{"id":"W1930968","doi":"10.2144/oct0211","title":"Isolation of Acetylcholinesterase from Apoptotic Human Lung Fibroblast Cells by Antibody Affinity Chromatography","year":2002,"lang":"en","type":"article","venue":"BioTechniques","topic":"Cholinesterase and Neurodegenerative Diseases","field":"Medicine","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute for Biological Sciences","funders":"National Cancer Institute; National Institutes of Health; National Natural Science Foundation of China","keywords":"Acetylcholinesterase; Affinity chromatography; Cholinergic; Apoptosis; Molecular biology; Cell culture; Aché; Biology; Tacrine; Fibroblast; Acetylcholine; Biochemistry; Chemistry; Enzyme; Cell biology; In vitro; Pharmacology; Endocrinology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002619377,0.000647212,0.0005301241,0.0005618238,0.0003150657,0.0003091797,0.0004754461,0.000428936,0.001355249],"category_scores_gemma":[0.0004024539,0.0001606153,0.000372871,0.0003691187,0.0001680822,0.0001929762,0.0002216593,0.0008013307,0.001811063],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002408145,"about_ca_system_score_gemma":0.0002684526,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001374202,"about_ca_topic_score_gemma":0.001964247,"domain_scores_codex":[0.9997683,0.00002865443,0.00002385543,0.00004365398,0.00007963765,0.00005598241],"domain_scores_gemma":[0.9997528,0.00008064956,0.00002091622,0.00002308384,0.00008742463,0.0000350026],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003923589,0.0000299331,0.0001539776,0.00005556224,0.000006082989,0.0001268009,0.00002247683,0.00002562805,0.995416,0.000056077,0.00008946537,0.003978653],"study_design_scores_gemma":[0.00006066197,0.0003855944,0.006746474,0.00002968243,0.00005770399,0.001198417,0.00004470976,0.001624407,0.9786684,0.0001848794,0.01098039,0.00001862017],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7464595,0.01983226,0.2176558,0.0009582165,0.000382971,0.0009729725,0.001649976,0.0007885608,0.01129976],"genre_scores_gemma":[0.7988517,0.02518844,0.1458769,0.0009495915,0.0002371675,0.0006556016,0.008184839,0.0002082902,0.01984745],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001374202,"threshold_uncertainty_score":0.004533827,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02004566796276125,"score_gpt":0.2993067561234479,"score_spread":0.2792610881606866,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}