{"id":"W1939988704","doi":"10.1111/j.1365-2958.2004.04159.x","title":"Activity reversal of Tet repressor caused by single amino acid exchanges","year":2004,"lang":"en","type":"article","venue":"Molecular Microbiology","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":87,"is_retracted":false,"has_abstract":true,"ca_institutions":"Vancouver Hospital and Health Sciences Centre; University of British Columbia","funders":"Deutsche Forschungsgemeinschaft","keywords":"Repressor; TetR; Biology; Allosteric regulation; Mutagenesis; Mutant; Effector; Mutation; Hydrogen bond; Inducer; DNA; Genetics; Amino acid; Biophysics; Biochemistry; Gene; Chemistry; Gene expression; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001147563,0.0003408728,0.0002878392,0.0001814416,0.0001110126,0.0002431332,0.0002343107,0.0002763831,0.001005777],"category_scores_gemma":[0.0003084454,0.0001396539,0.000280043,0.0001232578,0.0002744309,0.0001088919,0.0001981961,0.0004817797,0.0002936838],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001674219,"about_ca_system_score_gemma":0.0001632921,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002947879,"about_ca_topic_score_gemma":0.0003385323,"domain_scores_codex":[0.9998505,0.00003258768,0.00001769286,0.00002279761,0.00004863512,0.00002786686],"domain_scores_gemma":[0.9997978,0.00006641567,0.0000625176,0.00003075735,0.00001274511,0.00002970655],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009352421,0.00003453871,0.0002034022,0.00001935197,0.000008633065,0.0001346363,0.00001102413,0.000383625,0.9974781,0.0001461464,0.0000115453,0.001475466],"study_design_scores_gemma":[0.00002082791,0.0003538191,0.001063445,0.000005047368,0.00001978087,0.0005206472,0.00001418859,0.001787851,0.995437,0.00007001903,0.0007000787,0.00000718478],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965529,0.0001742377,0.002438858,0.00004154242,0.00001318732,0.00002388146,0.00006411393,0.00006692459,0.0006243263],"genre_scores_gemma":[0.9980756,0.0001164992,0.001365075,0.00001779037,0.000002947795,0.00001044111,0.00008181353,0.0000229438,0.0003068284],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001005777,"threshold_uncertainty_score":0.003364623,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006743232378488119,"score_gpt":0.2120332099903512,"score_spread":0.2052899776118631,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}