{"id":"W1939988704","doi":"10.1111/j.1365-2958.2004.04159.x","title":"Activity reversal of Tet repressor caused by single amino acid exchanges","year":2004,"lang":"en","type":"article","venue":"Molecular Microbiology","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":87,"is_retracted":false,"has_abstract":true,"ca_institutions":"Vancouver Hospital and Health Sciences Centre; University of British Columbia","funders":"Deutsche Forschungsgemeinschaft","keywords":"Repressor; TetR; Biology; Allosteric regulation; Mutagenesis; Mutant; Effector; Mutation; Hydrogen bond; Inducer; DNA; Genetics; Amino acid; Biophysics; Biochemistry; Gene; Chemistry; Gene expression; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009642509,0.0001905216,0.0003072848,0.00003766739,0.00003507921,0.000007819708,0.0002292523,0.0002859735,0.00004271123],"category_scores_gemma":[0.0001019021,0.000179555,0.000212253,0.00009917314,0.0001876825,0.000002569575,0.0001566763,0.00008643975,0.000008176727],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002815669,"about_ca_system_score_gemma":0.00002561759,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006869499,"about_ca_topic_score_gemma":0.00002399528,"domain_scores_codex":[0.9988958,0.00007958089,0.000204424,0.0004840104,0.00004922587,0.0002870104],"domain_scores_gemma":[0.9992735,0.000009720473,0.0001414926,0.0004515738,0.00005885726,0.00006482056],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004440478,0.0002017546,0.00009286047,0.00001538975,0.0001438154,0.000006780328,0.00001477286,0.00001024667,0.9977154,0.00000950563,0.0009859218,0.0007591957],"study_design_scores_gemma":[0.0005115922,0.000191685,0.00005281555,0.0000117484,0.00005660707,0.00001455574,0.000009763432,5.696175e-7,0.9823602,0.00003243892,0.01656116,0.0001967994],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9945694,0.001482443,0.002976444,0.0003961508,0.00004291528,0.00009648793,0.0001294916,0.00001233163,0.0002943199],"genre_scores_gemma":[0.9986691,0.00007054539,0.000439931,0.0002824923,0.00004102142,0.0000102444,0.0002834183,0.0000228449,0.0001804266],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01557524,"threshold_uncertainty_score":0.7322042,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006743232378488119,"score_gpt":0.2120332099903512,"score_spread":0.2052899776118631,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}