{"id":"W195141079","doi":"10.1096/fasebj.21.6.a825-a","title":"Construction of a Bicistronic Lentiviral Vector for Efficient Transduction and Expression of Multiple Therapeutic Genes in Adult Stem Cells","year":2007,"lang":"en","type":"article","venue":"The FASEB Journal","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University","funders":"Canadian Institutes of Health Research","keywords":"Internal ribosome entry site; Transduction (biophysics); Reporter gene; Green fluorescent protein; Biology; Luciferase; Viral vector; Molecular biology; HEK 293 cells; Plasmid; Gene; Transfection; Expression vector; Genetic enhancement; Translation (biology); Cell biology; Gene expression; Recombinant DNA; Messenger RNA; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009884476,0.0007280729,0.0007664585,0.0006915636,0.0003624145,0.0007400421,0.0008404548,0.0006739705,0.001728107],"category_scores_gemma":[0.0003686907,0.0005427279,0.0005099684,0.0006400125,0.0004521686,0.0004371493,0.0005497335,0.001612882,0.001601859],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005686656,"about_ca_system_score_gemma":0.0005593261,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005466145,"about_ca_topic_score_gemma":0.0006241156,"domain_scores_codex":[0.9990546,0.0001200078,0.0001327787,0.0001847939,0.0003829891,0.0001247572],"domain_scores_gemma":[0.9997122,0.00004382154,0.00005568232,0.00005686406,0.00006959089,0.000061876],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005482795,0.00006395352,0.0001139272,0.00008472465,0.00000813246,0.00007424768,0.00003890881,0.0002251881,0.9949568,0.0006596484,0.0002521405,0.003467527],"study_design_scores_gemma":[0.00005704886,0.0003436963,0.0009833621,0.00002107355,0.00004136038,0.0003594976,0.00002665346,0.005457257,0.9777077,0.0001509126,0.01483295,0.00001851607],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5351391,0.003642968,0.4383204,0.000466452,0.0007128039,0.003243931,0.00725715,0.002486829,0.008730259],"genre_scores_gemma":[0.6771142,0.00282389,0.2782722,0.0003492577,0.0001092107,0.002918913,0.01574477,0.0004989763,0.02216848],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001728107,"threshold_uncertainty_score":0.005781114,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01741817277155703,"score_gpt":0.273675753062627,"score_spread":0.25625758029107,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}