{"id":"W1963689503","doi":"10.1107/s0907444902023478","title":"Expression, purification, crystallization and preliminary crystallographic analysis of<i>Trypanosoma brucei</i>phosphofructokinase","year":2003,"lang":"en","type":"article","venue":"Acta Crystallographica Section D Biological Crystallography","topic":"Biochemical and Molecular Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"","keywords":"Molecular replacement; Chemistry; Escherichia coli; Trypanosoma brucei; Phosphofructokinase; Allosteric regulation; Orthorhombic crystal system; Crystallography; Enzyme; Biochemistry; Crystal structure; Glycolysis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003153479,0.0004420055,0.0005304666,0.0002584493,0.0004736109,0.0005354117,0.0004368386,0.000248209,0.001070942],"category_scores_gemma":[0.0005932435,0.0002672725,0.0003101025,0.001020247,0.0002359151,0.000237735,0.0002001562,0.0008105259,0.0009757999],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001128827,"about_ca_system_score_gemma":0.0005459798,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.009815228,"about_ca_topic_score_gemma":0.007937934,"domain_scores_codex":[0.9997601,0.00003695688,0.00003552766,0.00004374239,0.00006399635,0.00005967101],"domain_scores_gemma":[0.9998249,0.00003043123,0.00001892356,0.00002886572,0.0000601821,0.0000367077],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003550233,0.0000875538,0.001196172,0.0002976859,0.00001598311,0.0004952893,0.0001929637,0.001194982,0.986676,0.0008265008,0.001204891,0.007457013],"study_design_scores_gemma":[0.00008540051,0.000246382,0.01104718,0.00003184854,0.00004367767,0.001224853,0.0001647272,0.003062412,0.9415782,0.0004758534,0.04200515,0.00003430147],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.949693,0.006569566,0.02026682,0.001266846,0.0001707108,0.0003784488,0.01358921,0.0004079655,0.007657463],"genre_scores_gemma":[0.8882126,0.009183513,0.04816901,0.0001987822,0.0000766669,0.0002073059,0.04200142,0.0002888898,0.01166175],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.009815228,"threshold_uncertainty_score":0.01951617,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01176371744759955,"score_gpt":0.2370815849233611,"score_spread":0.2253178674757615,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}