{"id":"W1963689503","doi":"10.1107/s0907444902023478","title":"Expression, purification, crystallization and preliminary crystallographic analysis of<i>Trypanosoma brucei</i>phosphofructokinase","year":2003,"lang":"en","type":"article","venue":"Acta Crystallographica Section D Biological Crystallography","topic":"Biochemical and Molecular Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"","keywords":"Molecular replacement; Chemistry; Escherichia coli; Trypanosoma brucei; Phosphofructokinase; Allosteric regulation; Orthorhombic crystal system; Crystallography; Enzyme; Biochemistry; Crystal structure; Glycolysis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0004686765,0.0006274844,0.0007553603,0.001513092,0.0003887549,0.00009263129,0.000511435,0.001001614,0.0001341488],"category_scores_gemma":[0.0004861599,0.0005401148,0.0009516714,0.004788433,0.001328054,0.00003657004,0.0002688979,0.0004242273,1.531698e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001521983,"about_ca_system_score_gemma":0.00007565087,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003069471,"about_ca_topic_score_gemma":0.00003303763,"domain_scores_codex":[0.9955036,0.0006761035,0.0009650562,0.001525094,0.0005556819,0.0007744461],"domain_scores_gemma":[0.9973761,0.0001230481,0.0004764026,0.001002582,0.0005599177,0.0004619923],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"observational","study_design_scores_codex":[0.0004640297,0.0004955305,0.05033682,0.00005721196,0.001126777,0.000006970556,0.00004033824,0.00004972568,0.9462694,0.0006302017,0.0003895125,0.0001334733],"study_design_scores_gemma":[0.003408694,0.005556401,0.4639034,0.0001023392,0.001721443,0.0001968954,0.0008010554,0.0001216193,0.2621637,0.003384799,0.2560104,0.002629275],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9833066,0.002653722,0.01188944,0.0001164492,0.0001404869,0.0007744257,0.0001081544,0.0001151199,0.0008955813],"genre_scores_gemma":[0.9934469,0.002901206,0.002125641,0.0002519629,0.00007160597,0.0001846208,0.0009138011,0.00005459208,0.00004959835],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.6841058,"threshold_uncertainty_score":0.999705,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01176371744759955,"score_gpt":0.2370815849233611,"score_spread":0.2253178674757615,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}