{"id":"W1963728028","doi":"10.1021/bi011518y","title":"Two Glutamate Residues, Glu 208α and Glu 197β, Are Crucial for Phosphorylation and Dephosphorylation of the Active-Site Histidine Residue in Succinyl-CoA Synthetase","year":2001,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta; Western University","funders":"National Center for Research Resources","keywords":"Dephosphorylation; Phosphorylation; Histidine; Biochemistry; Alanine; Residue (chemistry); Mutant; Chemistry; G alpha subunit; Serine; Protein subunit; Active site; Enzyme; Amino acid; Phosphatase","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005947939,0.0004268663,0.0002093634,0.00007743636,0.0001369245,0.0002271692,0.0001898383,0.0002353998,0.0009233038],"category_scores_gemma":[0.0001452231,0.000153451,0.0002125407,0.00009675528,0.0002357127,0.0001703387,0.0001694708,0.0003029076,0.0005080153],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002914984,"about_ca_system_score_gemma":0.0002603683,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007801882,"about_ca_topic_score_gemma":0.0006893359,"domain_scores_codex":[0.9999363,0.000008370709,0.000008444346,0.00001217252,0.00002042029,0.0000143218],"domain_scores_gemma":[0.9999059,0.00001337299,0.00003637007,0.000007803983,0.000008146696,0.00002838334],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005394447,0.00004526303,0.001571645,0.00005600999,0.00001381596,0.0005499331,0.0000236731,0.0003365625,0.9938501,0.0002716738,0.00003829134,0.002703421],"study_design_scores_gemma":[0.00009087087,0.0002679213,0.01504645,0.00001427314,0.00004126051,0.001456773,0.00009028658,0.001222994,0.9796069,0.0001842865,0.001965998,0.00001202331],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998081,0.0001797946,0.001146624,0.00004133023,0.00001554707,0.00001039753,0.00008764142,0.00002193538,0.0004156763],"genre_scores_gemma":[0.9977595,0.0002017675,0.001163539,0.00003194558,0.000005245809,0.00000851003,0.0002718795,0.000009656729,0.0005479683],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009233038,"threshold_uncertainty_score":0.003088772,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007605580335911885,"score_gpt":0.2267937976705302,"score_spread":0.2191882173346183,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}