{"id":"W1964151866","doi":"10.1038/ncomms4283","title":"Reconstitution of a 10-gene pathway for synthesis of the plant alkaloid dihydrosanguinarine in Saccharomyces cerevisiae","year":2014,"lang":"en","type":"article","venue":"Nature Communications","topic":"Berberine and alkaloids research","field":"Medicine","cited_by":186,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary; Concordia University","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; Genome Alberta; Government of Alberta; Genome Canada","keywords":"Benzylisoquinoline; Sanguinarine; Saccharomyces cerevisiae; Alkaloid; Yeast; Biology; Biochemistry; Gene; Chemistry; Biosynthesis; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002249665,0.0006602656,0.0003947709,0.0002884461,0.0001953771,0.0004954993,0.0005547569,0.00036238,0.001391661],"category_scores_gemma":[0.0001676491,0.0003067059,0.0004820323,0.0002900065,0.0002852703,0.0002788502,0.00043246,0.0009221925,0.001016837],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008149563,"about_ca_system_score_gemma":0.0006342587,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004046955,"about_ca_topic_score_gemma":0.003729884,"domain_scores_codex":[0.9998409,0.00002432239,0.00002572602,0.00003964519,0.00003840499,0.00003104613],"domain_scores_gemma":[0.9998392,0.0000416618,0.00003248461,0.00003303086,0.00001461512,0.00003883822],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001797187,0.00008044681,0.0001732809,0.00005782796,0.00001040821,0.00006827042,0.00003893772,0.000275454,0.9974469,0.0003463854,0.00007705686,0.001245289],"study_design_scores_gemma":[0.00004801721,0.0001389942,0.001278312,0.0000141653,0.00002411735,0.0001160942,0.00006629814,0.002311674,0.9910252,0.0001171829,0.004845749,0.00001428569],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9797853,0.0008049119,0.01258897,0.0002606528,0.0001316324,0.0001341334,0.003507318,0.0006873348,0.002099875],"genre_scores_gemma":[0.9738974,0.0006272582,0.01218162,0.00007309717,0.00001068185,0.00007953085,0.007073789,0.0002736691,0.005782926],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004046955,"threshold_uncertainty_score":0.008046806,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03507885262060445,"score_gpt":0.3144770541619161,"score_spread":0.2793982015413116,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}