{"id":"W1964151866","doi":"10.1038/ncomms4283","title":"Reconstitution of a 10-gene pathway for synthesis of the plant alkaloid dihydrosanguinarine in Saccharomyces cerevisiae","year":2014,"lang":"en","type":"article","venue":"Nature Communications","topic":"Berberine and alkaloids research","field":"Medicine","cited_by":186,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary; Concordia University","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; Genome Alberta; Government of Alberta; Genome Canada","keywords":"Benzylisoquinoline; Sanguinarine; Saccharomyces cerevisiae; Alkaloid; Yeast; Biology; Biochemistry; Gene; Chemistry; Biosynthesis; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005031425,0.00008412651,0.0002760938,0.0001217338,0.00008410259,0.000004597514,0.0005821864,0.0001518882,0.00003276885],"category_scores_gemma":[0.001649749,0.00005749755,0.0001200901,0.0003492052,0.0002218159,0.00003869358,0.0002129693,0.0004023865,0.000002329563],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003320715,"about_ca_system_score_gemma":0.0001279085,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008607326,"about_ca_topic_score_gemma":0.0004763217,"domain_scores_codex":[0.9991378,0.0001338201,0.0003184961,0.000118713,0.0001621493,0.0001290769],"domain_scores_gemma":[0.9974716,0.0007705807,0.0001398826,0.00138904,0.0001913257,0.00003752987],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007528379,0.002497116,0.09701685,0.001436669,0.0003414864,0.000001789279,0.0007564292,0.00003525232,0.6783896,0.1147585,0.009116888,0.09489654],"study_design_scores_gemma":[0.0019839,0.0003426798,0.09097525,0.001321434,0.0001557474,0.00004850511,0.0001592606,0.002604846,0.5893185,0.0008461312,0.3120098,0.0002339411],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9338707,0.01013155,0.0001528914,0.02279605,0.0001619514,0.002389271,0.0006437461,0.00005047,0.0298034],"genre_scores_gemma":[0.9956145,0.0005020875,0.003332359,0.00007353558,0.00003284718,0.0001131045,0.00007369071,0.000009558524,0.0002483497],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3028929,"threshold_uncertainty_score":0.2344682,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03507885262060445,"score_gpt":0.3144770541619161,"score_spread":0.2793982015413116,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}