{"id":"W1965017622","doi":"10.1002/jgm.1370","title":"Development of a scalable process for high‐yield lentiviral vector production by transient transfection of HEK293 suspension cultures","year":2009,"lang":"en","type":"article","venue":"The Journal of Gene Medicine","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":142,"is_retracted":false,"has_abstract":true,"ca_institutions":"Polytechnique Montréal; National Research Council Canada; Biotechnology Research Institute","funders":"","keywords":"Transfection; HEK 293 cells; Transient (computer programming); Yield (engineering); Suspension (topology); Process (computing); Scalability; Viral vector; Production (economics); Cell culture; Computer science; Cell biology; Chemistry; Materials science; Biology; Genetics; Mathematics; Recombinant DNA; Biochemistry; Gene; Operating system","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00112613,0.0001061734,0.0002423928,0.00006420678,0.00006590108,0.000001730192,0.0001602874,0.00006143818,0.00001278953],"category_scores_gemma":[0.00008344655,0.00006268065,0.00006007004,0.0001290276,0.00009025799,0.000006483446,0.000004006725,0.00009555453,1.297858e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001915782,"about_ca_system_score_gemma":0.0001268973,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000006106085,"about_ca_topic_score_gemma":0.00003682432,"domain_scores_codex":[0.9988618,0.00006999477,0.0004522498,0.0001107674,0.0003602178,0.0001449319],"domain_scores_gemma":[0.9991456,0.00001381868,0.0002322977,0.0001371527,0.0004257345,0.00004543212],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.006278311,0.0001644945,0.00001150766,0.00005830144,0.00008114882,2.479785e-7,0.001032935,0.0002128585,0.9822132,0.000001175589,0.001769462,0.008176381],"study_design_scores_gemma":[0.001223808,0.008589276,0.000427914,0.000114584,0.00006690092,0.00002638098,0.0003152394,0.000008336699,0.9885532,0.00003839689,0.0005751367,0.00006085406],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9905761,0.002413704,0.004649333,0.001865331,0.0001422685,0.0003305085,0.000003571144,0.000001915592,0.00001722359],"genre_scores_gemma":[0.9987106,0.0004880393,0.0003084772,0.0000583863,0.000287148,0.000003582363,0.00001716019,0.000009658776,0.0001169904],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008424782,"threshold_uncertainty_score":0.2556043,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02210646035186192,"score_gpt":0.3129955088183266,"score_spread":0.2908890484664646,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}