{"id":"W1965035332","doi":"10.1016/j.chembiol.2012.01.006","title":"A Fluorogenic Red Fluorescent Protein Heterodimer","year":2012,"lang":"en","type":"article","venue":"Chemistry & Biology","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":100,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; University of Alberta","keywords":"Aequorea victoria; Green fluorescent protein; Fluorescence; Biosensor; Calmodulin; Bimolecular fluorescence complementation; Protein engineering; Fluorescent protein; Directed evolution; Biology; Biophysics; Biochemistry; Live cell imaging; Intracellular; Rational design; Cell biology; Cell; Enzyme; Gene; Mutant; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009232479,0.0006716595,0.0004418527,0.0004761751,0.0005164746,0.0007484704,0.000758503,0.0009235787,0.004295792],"category_scores_gemma":[0.0003727308,0.0003541179,0.0004221081,0.0005348361,0.0003948744,0.0005597213,0.0005591015,0.001633262,0.002375537],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006590315,"about_ca_system_score_gemma":0.0003283522,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003072752,"about_ca_topic_score_gemma":0.0002293761,"domain_scores_codex":[0.9993843,0.0001160992,0.00003774412,0.0002219782,0.0001343498,0.0001054168],"domain_scores_gemma":[0.9997141,0.00005168785,0.00003139925,0.00007315088,0.00003360067,0.00009607885],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002381822,0.0001206641,0.0002458074,0.00005927246,0.00001407936,0.00009565995,0.00003352793,0.00007431342,0.9936349,0.001691737,0.00113631,0.00265554],"study_design_scores_gemma":[0.00003440706,0.0001237217,0.0009377755,0.000008972365,0.0000210797,0.000408494,0.00002997619,0.0007501098,0.9814832,0.0002260186,0.01596709,0.000009259298],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8771691,0.002142922,0.08269187,0.00143378,0.0007670124,0.0003005973,0.002699336,0.002534325,0.03026112],"genre_scores_gemma":[0.926179,0.001535431,0.02835205,0.0006531504,0.0000945366,0.0002684825,0.007223142,0.0003747595,0.03531957],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004295792,"threshold_uncertainty_score":0.01437086,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008712420931569908,"score_gpt":0.276085574055943,"score_spread":0.267373153124373,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}