{"id":"W1965044238","doi":"10.1021/bi7018173","title":"Core Residue Replacements Cause Coiled-Coil Orientation Switching<i>in Vitro</i>and<i>in Vivo</i>:  Structure−Function Correlations for Osmosensory Transporter ProP","year":2007,"lang":"en","type":"article","venue":"Biochemistry","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"National Institute of Allergy and Infectious Diseases","keywords":"Antiparallel (mathematics); Coiled coil; Heptad repeat; Biophysics; Mutant; Chemistry; Transmembrane domain; Biochemistry; Crystallography; Peptide sequence; Biology; Amino acid; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001628845,0.0002388483,0.0001746832,0.0001120681,0.00009986382,0.0002066458,0.0001945078,0.0003222832,0.0009466328],"category_scores_gemma":[0.0002366982,0.0001294221,0.0002303569,0.0001382514,0.0002035905,0.0001300032,0.0001447758,0.0004735152,0.0002755766],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003403239,"about_ca_system_score_gemma":0.0001224835,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009058944,"about_ca_topic_score_gemma":0.000780636,"domain_scores_codex":[0.9998504,0.00003582847,0.00002161037,0.00002341497,0.00003346378,0.00003528686],"domain_scores_gemma":[0.9996991,0.0000875535,0.00009628342,0.00002953186,0.00002519582,0.00006237952],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001592902,0.00003782247,0.0003170841,0.00001206525,0.000006827371,0.00009553561,0.000009315825,0.0001177459,0.9988549,0.00004365088,0.00003613378,0.0003096349],"study_design_scores_gemma":[0.00001646536,0.0002370359,0.00454243,0.000002959924,0.00001272492,0.0003655577,0.00002383799,0.001762386,0.9924677,0.00002463048,0.0005379636,0.000006289797],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998848,0.0001312719,0.0005133431,0.00003498332,0.00001063834,0.00000836988,0.0001645126,0.0000236931,0.0002651699],"genre_scores_gemma":[0.9983608,0.00008845254,0.0005051654,0.00004317811,0.00000416157,0.000009201184,0.0005097136,0.00002724784,0.0004520824],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009466328,"threshold_uncertainty_score":0.003166854,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01338121113736295,"score_gpt":0.25485230503907,"score_spread":0.241471093901707,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}