{"id":"W1965131678","doi":"10.1021/bi034939j","title":"Creation of a Fully Functional Cysteine-Less Variant of Osmosensor and Proton-Osmoprotectant Symporter ProP from <i>Escherichia coli </i>and Its Application to Assess the Transporter's Membrane Orientation","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Amino Acid Enzymes and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; Hospital for Sick Children; University of Toronto","funders":"","keywords":"Symporter; Escherichia coli; Transporter; Cysteine; Chemistry; Biochemistry; Osmoprotectant; Proton; Biophysics; Cell biology; Biology; Gene; Enzyme; Proline; Amino acid; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001713913,0.0001458378,0.0001711036,0.00001521193,0.00004859274,0.000009980371,0.00007300423,0.0001412623,0.000007986371],"category_scores_gemma":[0.0000450744,0.0001150667,0.00004047007,0.00009162605,0.00006584354,0.000006127234,0.00002103127,0.00005784452,3.268757e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006833456,"about_ca_system_score_gemma":0.00008425,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002695385,"about_ca_topic_score_gemma":0.000003382384,"domain_scores_codex":[0.9990624,0.00003611068,0.0002913689,0.0003351351,0.0001584754,0.0001165178],"domain_scores_gemma":[0.9993392,0.00001053599,0.0001892687,0.0002415247,0.0001522542,0.00006718553],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001603511,0.00005987702,0.0008020268,0.0001510523,0.00004759311,6.455555e-7,0.00008775948,0.00001528185,0.9981322,0.0002093128,0.00002019021,0.0003136747],"study_design_scores_gemma":[0.0005914494,0.00008647721,0.005161215,0.00002653436,0.00006454497,0.00001121239,0.0001526226,0.00008049652,0.9914976,0.00002578213,0.002172233,0.0001298793],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9910514,0.0005839153,0.007049376,0.00007217773,0.00004307959,0.0008673465,0.00009936954,0.000005748497,0.0002275307],"genre_scores_gemma":[0.9984021,0.00007228889,0.0006811296,0.00006335274,0.00008816162,0.000286846,0.0001989009,0.00001492434,0.0001923269],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007350613,"threshold_uncertainty_score":0.4692284,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01064271137114956,"score_gpt":0.223381376647124,"score_spread":0.2127386652759745,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}