{"id":"W1965131678","doi":"10.1021/bi034939j","title":"Creation of a Fully Functional Cysteine-Less Variant of Osmosensor and Proton-Osmoprotectant Symporter ProP from <i>Escherichia coli </i>and Its Application to Assess the Transporter's Membrane Orientation","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Amino Acid Enzymes and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; Hospital for Sick Children; University of Toronto","funders":"","keywords":"Symporter; Escherichia coli; Transporter; Cysteine; Chemistry; Biochemistry; Osmoprotectant; Proton; Biophysics; Cell biology; Biology; Gene; Enzyme; Proline; Amino acid; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001888783,0.0004171756,0.0003179038,0.0001734201,0.0001304246,0.0003844243,0.0005056934,0.0003874443,0.000617045],"category_scores_gemma":[0.0002440101,0.0001813674,0.0003007244,0.0003114229,0.000307468,0.0001954914,0.0003186086,0.0006948668,0.0003999736],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003115833,"about_ca_system_score_gemma":0.0002433073,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007806133,"about_ca_topic_score_gemma":0.000765297,"domain_scores_codex":[0.9998298,0.00002643581,0.00002807764,0.00003066139,0.00005891158,0.00002607659],"domain_scores_gemma":[0.9997568,0.00003155112,0.00009214793,0.00003059922,0.00003488759,0.00005405285],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001047442,0.0000229357,0.0001246081,0.0000269641,0.00000972468,0.00008293235,0.00001291279,0.0001083589,0.9987292,0.000114209,0.00002947413,0.0006338934],"study_design_scores_gemma":[0.00002303985,0.0002259048,0.002769427,0.000006244305,0.00002690525,0.0006308207,0.00003468293,0.001203416,0.9921181,0.00003565012,0.002910584,0.00001534251],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9902289,0.0002241535,0.007421338,0.0001017817,0.0000392434,0.0000870931,0.001196153,0.00009596033,0.0006054576],"genre_scores_gemma":[0.9806189,0.0003009563,0.01272373,0.00007240689,0.00001361183,0.00007107586,0.003896191,0.000121427,0.002181782],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007806133,"threshold_uncertainty_score":0.002260685,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01064271137114956,"score_gpt":0.223381376647124,"score_spread":0.2127386652759745,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}