{"id":"W1965355331","doi":"10.1111/j.1365-2672.2007.03674.x","title":"Rapid detection and identification of the bacterium Pantoea stewartii in maize by TaqMan<sup>®</sup>real-time PCR assay targeting the cpsD gene","year":2008,"lang":"en","type":"article","venue":"Journal of Applied Microbiology","topic":"Plant Pathogenic Bacteria Studies","field":"Agricultural and Biological Sciences","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"Public Health Agency of Canada; Agriculture and Agri-Food Canada","funders":"Agriculture and Agri-Food Canada; Society for Applied Microbiology","keywords":"TaqMan; Pantoea; Polymerase chain reaction; Real-time polymerase chain reaction; Subcloning; Biology; Molecular biology; Serial dilution; Microbiology; Bacteria; Chromatography; Escherichia coli; Gene; Chemistry; 16S ribosomal RNA; Genetics; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000912879,0.0001296155,0.0002951454,0.00001954085,0.000211307,0.00001418489,0.0002633827,0.0001185246,0.0000371139],"category_scores_gemma":[0.00004753939,0.00004260518,0.00008074105,0.0001759472,0.0002111862,0.00005693109,0.000101846,0.0001903633,0.000005233115],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003113645,"about_ca_system_score_gemma":0.00001154111,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002351036,"about_ca_topic_score_gemma":0.00002907098,"domain_scores_codex":[0.9987392,0.0002184131,0.0005968239,0.000166079,0.0000655091,0.0002139047],"domain_scores_gemma":[0.9989392,0.0002685506,0.0006398564,0.00005755654,0.00006687368,0.00002794675],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001067321,0.00003776879,0.0008267823,0.000003391126,0.00002894228,0.000001526733,0.0002675934,0.000007003959,0.9954634,0.000002251412,0.0005393095,0.002715355],"study_design_scores_gemma":[0.0003942248,0.000142816,0.1362523,0.00001305659,0.00003611228,0.0004939985,0.0004981609,0.00003116975,0.8558648,0.00004545327,0.006085829,0.0001421082],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9987658,0.0002915183,0.000004006164,0.0004929304,0.000103951,0.0001813325,0.00009784547,0.000006309392,0.00005636898],"genre_scores_gemma":[0.99885,0.0008401033,0.00005229429,0.00006581769,0.0001231253,0.00000557143,0.00002104832,0.000001422267,0.00004058831],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1395986,"threshold_uncertainty_score":0.1737389,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008478055612996138,"score_gpt":0.1695816055341781,"score_spread":0.161103549921182,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}