{"id":"W1965355331","doi":"10.1111/j.1365-2672.2007.03674.x","title":"Rapid detection and identification of the bacterium Pantoea stewartii in maize by TaqMan<sup>®</sup>real-time PCR assay targeting the cpsD gene","year":2008,"lang":"en","type":"article","venue":"Journal of Applied Microbiology","topic":"Plant Pathogenic Bacteria Studies","field":"Agricultural and Biological Sciences","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"Public Health Agency of Canada; Agriculture and Agri-Food Canada","funders":"Agriculture and Agri-Food Canada; Society for Applied Microbiology","keywords":"TaqMan; Pantoea; Polymerase chain reaction; Real-time polymerase chain reaction; Subcloning; Biology; Molecular biology; Serial dilution; Microbiology; Bacteria; Chromatography; Escherichia coli; Gene; Chemistry; 16S ribosomal RNA; Genetics; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008348166,0.0005948058,0.0003374879,0.0005353247,0.0001497903,0.0005081251,0.000425594,0.0006172589,0.0006560048],"category_scores_gemma":[0.0009201316,0.0003702547,0.0003080249,0.0004269341,0.0004134256,0.0003491281,0.000393554,0.0005235533,0.0005876424],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003941908,"about_ca_system_score_gemma":0.0003758706,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001254696,"about_ca_topic_score_gemma":0.002102277,"domain_scores_codex":[0.9988031,0.0001652751,0.00008616947,0.0003172909,0.0005127048,0.0001153492],"domain_scores_gemma":[0.9994317,0.0001611807,0.0001752443,0.00005447086,0.0001340012,0.00004332892],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004604838,0.00001344211,0.001138694,0.00004170683,0.000001840499,0.000019487,0.00001617824,0.00004334856,0.9966002,0.00002257881,0.00002049536,0.002036051],"study_design_scores_gemma":[0.00002338719,0.0008737245,0.02238844,0.00001867173,0.00003422016,0.0004990535,0.0001067748,0.002201919,0.9706043,0.00006535259,0.003165213,0.00001888138],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8994177,0.00238524,0.09311722,0.0002725094,0.00008332242,0.0005255107,0.00212287,0.0005740459,0.00150161],"genre_scores_gemma":[0.816685,0.002263395,0.172516,0.0001472695,0.00004213598,0.0004435114,0.00379693,0.0000647472,0.004040935],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001254696,"threshold_uncertainty_score":0.004415035,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008478055612996138,"score_gpt":0.1695816055341781,"score_spread":0.161103549921182,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}