{"id":"W1966819125","doi":"10.3791/3932","title":"Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates","year":2012,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; St. Michael's Hospital","funders":"Ontario Ministry of Research and Innovation; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Kidney Foundation of Canada","keywords":"Guanine nucleotide exchange factor; RHOA; Cell biology; GTPase; Biology; Mutant; GTPase-activating protein; G protein; Gene; Signal transduction; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004592488,0.0002322669,0.0003840643,0.0001200089,0.00009498835,0.00003435244,0.0001814797,0.0001966045,0.0001210391],"category_scores_gemma":[0.0001604059,0.0002080557,0.000237144,0.0001155813,0.00006943609,0.00004902976,0.00008693725,0.0001462238,0.000004226575],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007831548,"about_ca_system_score_gemma":0.000156959,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007239152,"about_ca_topic_score_gemma":0.000001069969,"domain_scores_codex":[0.9980555,0.0003384634,0.0007099026,0.000194586,0.000407025,0.0002945888],"domain_scores_gemma":[0.9981661,0.00002680264,0.001109296,0.0002080797,0.0002997568,0.0001898984],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001327773,0.0005859528,0.0002999782,0.00002163654,0.0001769951,0.000003227905,0.001343208,0.0000503951,0.9953336,0.00002520049,0.00001205629,0.0008200119],"study_design_scores_gemma":[0.003007563,0.0003809972,0.0005521839,0.0001446406,0.00007094207,0.00001067549,0.0007562541,0.0001503529,0.9939745,0.0001097622,0.0006253858,0.0002167103],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.993738,0.002174129,0.003252853,0.0000166571,0.0002566133,0.0003853102,0.00004884106,0.000006660561,0.0001209234],"genre_scores_gemma":[0.9884428,0.0000431261,0.01086932,0.00004127239,0.0004769616,0.00001405314,0.00004387185,0.00003368682,0.00003491563],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00761647,"threshold_uncertainty_score":0.8484266,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02869488271459325,"score_gpt":0.3707744601684837,"score_spread":0.3420795774538904,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}