{"id":"W1967494653","doi":"10.1016/j.ab.2006.09.017","title":"Variability in the immunodetection of His-tagged recombinant proteins","year":2006,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":50,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Ministrstvo za Izobraževanje, Znanost in Šport; World Anti-Doping Agency; National Aeronautics and Space Administration; U.S. Department of Defense; National Cancer Institute; National Institutes of Health; National Science Foundation","keywords":"Chinese hamster ovary cell; Recombinant DNA; Affinity chromatography; Protein tag; Molecular biology; Chemistry; Myc-tag; Biochemistry; Monoclonal antibody; Antibody; Gel electrophoresis; Complementary DNA; Fusion protein; Biology; Gene; Enzyme; Receptor","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003856295,0.00008963121,0.000102376,0.00001277601,0.0000367936,0.00000856299,0.0001998527,0.000128092,0.00001779401],"category_scores_gemma":[0.00006874237,0.00006623951,0.00008370431,0.0001390838,0.0001021594,0.000001485104,0.0000213799,0.0001049473,0.000002446279],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001264888,"about_ca_system_score_gemma":0.00004622339,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001377216,"about_ca_topic_score_gemma":0.00002392565,"domain_scores_codex":[0.9992325,0.00004718185,0.0002448053,0.0002311842,0.00009981481,0.0001445126],"domain_scores_gemma":[0.9994797,0.00002742563,0.00005183437,0.000388064,0.00003362211,0.00001938283],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003262452,0.0002606684,0.002192037,0.00002093061,0.00001200454,7.395786e-7,0.000004983502,0.000008573637,0.9959974,0.0004771475,0.0004932732,0.0004995695],"study_design_scores_gemma":[0.0003543936,0.00005200966,0.01624028,0.00001089037,0.0000241551,0.00001238098,0.00003391748,0.0001854873,0.9778369,0.0009033625,0.004219629,0.0001266029],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9940556,0.00008450509,0.001441187,0.0003493479,0.000017152,0.0001524054,0.00002553397,0.00000565019,0.003868634],"genre_scores_gemma":[0.999289,0.00001601267,0.0001608557,0.00003847224,0.00005836033,0.00003455768,0.0001217014,0.000005180861,0.0002758001],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01816055,"threshold_uncertainty_score":0.2701169,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005610391899716664,"score_gpt":0.2285999901321817,"score_spread":0.222989598232465,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}