{"id":"W1967494653","doi":"10.1016/j.ab.2006.09.017","title":"Variability in the immunodetection of His-tagged recombinant proteins","year":2006,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":50,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Ministrstvo za Izobraževanje, Znanost in Šport; World Anti-Doping Agency; National Aeronautics and Space Administration; U.S. Department of Defense; National Cancer Institute; National Institutes of Health; National Science Foundation","keywords":"Chinese hamster ovary cell; Recombinant DNA; Affinity chromatography; Protein tag; Molecular biology; Chemistry; Myc-tag; Biochemistry; Monoclonal antibody; Antibody; Gel electrophoresis; Complementary DNA; Fusion protein; Biology; Gene; Enzyme; Receptor","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003620349,0.0005852947,0.0008680054,0.0009825059,0.000352034,0.0014288,0.0006182253,0.0006310236,0.0009324147],"category_scores_gemma":[0.005204248,0.0005656295,0.0007710316,0.000902684,0.0005612893,0.0004713224,0.0009403547,0.001901479,0.0007605119],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005291702,"about_ca_system_score_gemma":0.0002203535,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004904262,"about_ca_topic_score_gemma":0.001007686,"domain_scores_codex":[0.9963346,0.0007836104,0.0006447826,0.0007771906,0.001129993,0.000329826],"domain_scores_gemma":[0.9892417,0.006096589,0.0006597122,0.002623675,0.001129595,0.0002487195],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005528625,0.00003142417,0.000592991,0.00004275777,0.00002908343,0.00004762779,0.00004409932,0.0001776831,0.9965879,0.0001503543,0.00005873551,0.002182059],"study_design_scores_gemma":[0.000006164368,0.00008740891,0.009045658,0.000009670851,0.00005416908,0.0004991838,0.00003239516,0.001450341,0.9861429,0.0003494876,0.002307687,0.00001487329],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.840555,0.003048881,0.1466814,0.0003738494,0.0002798826,0.0002366221,0.001563668,0.001505478,0.00575518],"genre_scores_gemma":[0.9588149,0.001208124,0.02984707,0.000471164,0.00006567332,0.0003319336,0.003959244,0.001042694,0.004259113],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003620349,"threshold_uncertainty_score":0.01914644,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005610391899716664,"score_gpt":0.2285999901321817,"score_spread":0.222989598232465,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}