{"id":"W1967882148","doi":"10.1042/0264-6021:3610653","title":"Cell-permeable ceramides preferentially inhibit coated vesicle formation and exocytosis in Chinese hamster ovary compared with Madin‒Darby canine kidney cells by preventing the membrane association of ADP-ribosylation factor","year":2002,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Sphingolipid Metabolism and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Fondation pour la Recherche Médicale; Canadian Diabetes Association; Heart and Stroke Foundation of Canada","keywords":"Chinese hamster ovary cell; Phospholipase D; Golgi apparatus; Cell biology; Ceramide; ADP ribosylation factor; Biology; Exocytosis; Cytosol; Molecular biology; Biochemistry; Membrane; Endoplasmic reticulum; Signal transduction; Apoptosis; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002502274,0.0001494844,0.0001943923,0.00004653893,0.00009200249,0.00006808349,0.0001106675,0.0001306606,0.00005302963],"category_scores_gemma":[0.00008031015,0.0001042063,0.00005168551,0.0001281838,0.00003185187,0.00003421769,0.000043533,0.000176398,8.336515e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003774468,"about_ca_system_score_gemma":0.0000276424,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002549174,"about_ca_topic_score_gemma":0.000007336285,"domain_scores_codex":[0.9988993,0.00009904094,0.0003904651,0.0001672713,0.0002284679,0.0002154826],"domain_scores_gemma":[0.999301,0.00002579826,0.0003545571,0.0001067569,0.0001119705,0.00009998969],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001020807,0.00008260554,0.009611243,0.00004254708,0.00003196897,3.775305e-7,0.0002738792,0.00007688379,0.9871799,3.027885e-7,0.002477009,0.0001212308],"study_design_scores_gemma":[0.001662463,0.00007293947,0.002865454,0.00006464383,0.00003476159,0.00001200757,0.00005396433,0.00245037,0.9923112,0.000004660973,0.0003341661,0.000133336],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998423,0.0007945607,0.0003114484,0.0001204526,0.0000578833,0.0001616632,0.00004332767,0.000005648579,0.00008198625],"genre_scores_gemma":[0.9989703,0.0002935871,0.0002257281,0.00009824928,0.0001449528,0.000005214803,0.0001145963,0.00001517195,0.0001321636],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006745789,"threshold_uncertainty_score":0.4249408,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007706038699171298,"score_gpt":0.197541185267329,"score_spread":0.1898351465681577,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}