{"id":"W1968769252","doi":"10.1016/s0378-1097(03)00820-6","title":"Allelic exchange in<i>Francisella tularensis</i>using PCR products","year":2003,"lang":"en","type":"article","venue":"FEMS Microbiology Letters","topic":"Bacillus and Francisella bacterial research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":93,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Victoria","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health","keywords":"Francisella tularensis; Biology; Mutant; Gene; Francisella; Polymerase chain reaction; Genetics; Microbiology; Ampicillin; Bacteria; Virulence","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000582805,0.000354928,0.0003370052,0.0003308774,0.0002453068,0.0005395284,0.0005119621,0.0004015417,0.0008060168],"category_scores_gemma":[0.0007487925,0.0003768284,0.0004813656,0.0002056134,0.0003076644,0.0003549341,0.0005134507,0.001281827,0.001086297],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001928002,"about_ca_system_score_gemma":0.0001967217,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005332276,"about_ca_topic_score_gemma":0.0009581498,"domain_scores_codex":[0.9995493,0.0000859706,0.00006403291,0.0001002124,0.000134132,0.00006634182],"domain_scores_gemma":[0.9994798,0.0001953146,0.0001381098,0.00009406199,0.00006068375,0.00003209936],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003452173,0.00002875146,0.0002774536,0.00004178441,0.000005328695,0.0001161727,0.000056066,0.00006216301,0.9923689,0.0002592718,0.00006487898,0.006684536],"study_design_scores_gemma":[0.000008560944,0.0001510369,0.0009783951,0.00001257678,0.00002473046,0.0009577306,0.00005059826,0.0005889158,0.9928053,0.00008117031,0.00432699,0.0000139796],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6533248,0.001729425,0.338556,0.0004843648,0.00018951,0.0006212455,0.0006271974,0.001183488,0.003284105],"genre_scores_gemma":[0.7848916,0.002285838,0.2012861,0.0002472777,0.00005096214,0.0003731318,0.002958742,0.0004899919,0.0074163],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008060168,"threshold_uncertainty_score":0.003082156,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01782434978408389,"score_gpt":0.2392774724520346,"score_spread":0.2214531226679507,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}