{"id":"W1968839547","doi":"10.1007/s00284-009-9377-z","title":"Cloning, Overexpression, Purification, and Characterization of the Maleylacetate Reductase from Sphingobium chlorophenolicum Strain ATCC 53874","year":2009,"lang":"en","type":"article","venue":"Current Microbiology","topic":"Microbial bioremediation and biosurfactants","field":"Environmental Science","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"Environment and Climate Change Canada; University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada; Saskatchewan Health Research Foundation","keywords":"Escherichia coli; Recombinant DNA; Pentachlorophenol; Enzyme; Strain (injury); Cloning (programming); Reductase; Biochemistry; Biology; Affinity chromatography; Gene; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000374155,0.0004673686,0.0005002417,0.0004217536,0.0002756726,0.0004863043,0.0004548612,0.0002665084,0.0004505921],"category_scores_gemma":[0.0004098639,0.0002638331,0.0004235852,0.0007173076,0.0002282232,0.0002756733,0.0004356624,0.0006680666,0.0005867626],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006729355,"about_ca_system_score_gemma":0.0007282631,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.008721346,"about_ca_topic_score_gemma":0.00674291,"domain_scores_codex":[0.9996359,0.00004876135,0.0000444933,0.00004112133,0.0001630677,0.00006664384],"domain_scores_gemma":[0.9997665,0.00004881514,0.0000443655,0.00004312327,0.0000588508,0.00003837336],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008904812,0.00004492959,0.0002801351,0.00004318526,0.000007218668,0.00005673213,0.00003926551,0.00008157639,0.9970217,0.0001063014,0.00007536198,0.002154531],"study_design_scores_gemma":[0.00002145284,0.0001062062,0.007234912,0.000007532451,0.00002926984,0.0002624423,0.00007065755,0.0009585689,0.9864818,0.0000762464,0.004736469,0.00001450007],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9857551,0.001136189,0.007184958,0.0005813029,0.00005047088,0.00008245045,0.003124348,0.0002160153,0.001869412],"genre_scores_gemma":[0.9677314,0.001423376,0.01127302,0.00009023516,0.00002352497,0.00006204948,0.0119456,0.0001399977,0.007310903],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008721346,"threshold_uncertainty_score":0.0173412,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008827338419746639,"score_gpt":0.21692160770291,"score_spread":0.2080942692831634,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}