{"id":"W1968839547","doi":"10.1007/s00284-009-9377-z","title":"Cloning, Overexpression, Purification, and Characterization of the Maleylacetate Reductase from Sphingobium chlorophenolicum Strain ATCC 53874","year":2009,"lang":"en","type":"article","venue":"Current Microbiology","topic":"Microbial bioremediation and biosurfactants","field":"Environmental Science","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"Environment and Climate Change Canada; University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada; Saskatchewan Health Research Foundation","keywords":"Escherichia coli; Recombinant DNA; Pentachlorophenol; Enzyme; Strain (injury); Cloning (programming); Reductase; Biochemistry; Biology; Affinity chromatography; Gene; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001093838,0.0001391156,0.0001485162,0.00002037315,0.00009589125,0.00001552289,0.0002353432,0.00009047623,0.0008413392],"category_scores_gemma":[0.00002787461,0.00009762781,0.00004086315,0.0001103436,0.000230836,0.00009234033,0.0001045313,0.0001215401,0.00006719583],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003873611,"about_ca_system_score_gemma":0.00001350384,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002701508,"about_ca_topic_score_gemma":0.0000094931,"domain_scores_codex":[0.9990455,0.00009719924,0.0002957351,0.0003162216,0.00005662522,0.0001887406],"domain_scores_gemma":[0.9993891,0.00001277872,0.0002766396,0.0002517607,0.00001564223,0.0000541109],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001715071,0.00006347571,0.005010563,0.000003555862,0.000003161724,1.085832e-7,0.0004234208,0.000001300254,0.9896443,0.00002929361,0.001215644,0.00358798],"study_design_scores_gemma":[0.0003234236,0.00003014077,0.2085315,0.00003740186,0.00001101206,0.000005572339,0.00001401442,0.00001431669,0.769682,0.0001106141,0.02109946,0.0001404555],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976512,0.00009051769,0.0000279126,0.0009680708,0.0003839695,0.0002355962,0.0005552701,0.00001804481,0.00006943063],"genre_scores_gemma":[0.998839,0.0001043798,0.000100221,0.000267018,0.00006578681,0.000001633479,0.0004899678,0.000005648062,0.0001263645],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2199623,"threshold_uncertainty_score":0.9212075,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008827338419746639,"score_gpt":0.21692160770291,"score_spread":0.2080942692831634,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}