{"id":"W1969235796","doi":"10.1039/b709817k","title":"Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays","year":2007,"lang":"en","type":"article","venue":"Chemical Communications","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":49,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"","keywords":"Oligonucleotide; DNA; DNA ligase; Rolling circle replication; Chemistry; Ligase chain reaction; DNA polymerase; Fluorescence; Sequencing by ligation; Ligation; Molecular biology; Biochemistry; Combinatorial chemistry; Polymerase chain reaction; Biology; Genomic library; Gene; Base sequence; Multiplex polymerase chain reaction","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003001922,0.0001464908,0.0002163162,0.00009089133,0.0001006874,0.000006197575,0.0005493381,0.0001510765,0.000001969425],"category_scores_gemma":[0.0001325697,0.0001372511,0.0001318802,0.0002210924,0.0003321378,0.000003425528,0.0003134359,0.0001124689,0.000002419924],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003270579,"about_ca_system_score_gemma":0.00006717871,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000008664021,"about_ca_topic_score_gemma":0.00003659171,"domain_scores_codex":[0.9989118,0.00003522135,0.0005564275,0.0001966912,0.000124817,0.0001750608],"domain_scores_gemma":[0.998289,0.00005805361,0.0002386593,0.001153506,0.0001982119,0.00006255278],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001898635,0.0002352011,0.0001854541,0.00002157317,0.00006794478,1.289609e-7,0.00006423605,5.591618e-7,0.9916187,0.000377045,0.0001290009,0.007281202],"study_design_scores_gemma":[0.0001326325,0.00003482191,0.0005865954,0.00005623834,0.00003494434,0.000003513995,0.0001067097,0.00001934994,0.9936566,0.0001835865,0.005041264,0.0001437872],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9924727,0.0003494358,0.004387391,0.0002005628,0.00001195837,0.0001401693,0.00002631907,0.00003511486,0.002376355],"genre_scores_gemma":[0.8883231,0.0001033386,0.1111307,0.00007500483,0.00001630103,0.000006071355,0.0002886941,0.00001559576,0.00004125008],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1067433,"threshold_uncertainty_score":0.5596939,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03395841445953653,"score_gpt":0.3161653091825734,"score_spread":0.2822068947230369,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}