{"id":"W1970226203","doi":"10.1016/j.pep.2003.10.003","title":"Expression and rapid purification of highly active hexahistidine-tagged guinea pig liver transglutaminase","year":2003,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Blood properties and coagulation","field":"Medicine","cited_by":25,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Montréal","funders":"Fonds Québécois de la Recherche sur la Nature et les Technologies","keywords":"Tissue transglutaminase; Recombinant DNA; Guinea pig; Escherichia coli; Enzyme; Substrate (aquarium); Biochemistry; Affinity chromatography; Chemistry; Chromatography; Molecular biology; Liver tissue; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005890751,0.0004252635,0.0004415163,0.0003135226,0.0002505809,0.0005752331,0.0004552454,0.0004823437,0.0006675177],"category_scores_gemma":[0.0004942368,0.0003660516,0.0004282956,0.0004719424,0.0003008302,0.0004140609,0.0004581794,0.001228364,0.0008909607],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000538415,"about_ca_system_score_gemma":0.0002953776,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001253272,"about_ca_topic_score_gemma":0.001307653,"domain_scores_codex":[0.9997032,0.00005677913,0.00004018333,0.00004763686,0.00008736237,0.00006476248],"domain_scores_gemma":[0.9996786,0.00006705336,0.00004374307,0.00008330073,0.00007833198,0.00004904489],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005546404,0.00001794083,0.00006381363,0.00003023345,0.000004698508,0.00004514117,0.00003235272,0.00006303405,0.9980806,0.0002052241,0.0001179924,0.00128351],"study_design_scores_gemma":[0.00002187772,0.00006318773,0.001213103,0.00000815623,0.00001683359,0.0002376151,0.00002784977,0.0009399567,0.990312,0.0001576803,0.006994598,0.00000708577],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8732106,0.005227185,0.1135933,0.001336997,0.0002769526,0.0003422546,0.002527494,0.0005517539,0.00293335],"genre_scores_gemma":[0.8769,0.00405369,0.0913506,0.0003019967,0.00007298472,0.0002258665,0.01004437,0.000261918,0.01678854],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001253272,"threshold_uncertainty_score":0.003906429,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02315997386789267,"score_gpt":0.238630427104396,"score_spread":0.2154704532365033,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}