{"id":"W1970617888","doi":"10.1371/journal.pone.0109213","title":"Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage","year":2014,"lang":"en","type":"article","venue":"PLoS ONE","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":119,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research","keywords":"CRISPR; Cas9; Genome editing; Nuclease; Biology; Computational biology; Palindrome; Guide RNA; Chromatin; DNA; In silico; Genetics; RNA; Subgenomic mRNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000214576,0.0001973278,0.000194903,0.00003222231,0.00009005082,0.00003383189,0.0001827062,0.0001317583,0.00006649799],"category_scores_gemma":[0.0001472816,0.000192286,0.00007315262,0.00006200837,0.00005663449,0.000004101293,0.0001039976,0.000158441,0.00004413747],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001492398,"about_ca_system_score_gemma":0.00002783514,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001843548,"about_ca_topic_score_gemma":0.0000307773,"domain_scores_codex":[0.9988158,0.00005656456,0.0001848996,0.0003765282,0.0002242734,0.000341894],"domain_scores_gemma":[0.9993474,0.00001325444,0.00004856718,0.0003973581,0.00005662262,0.0001368033],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002439265,0.0002627914,0.001887825,0.0001138823,0.0001003905,0.000003831499,0.0001022914,0.0001077583,0.9959208,0.00004607689,0.0009786383,0.0004513523],"study_design_scores_gemma":[0.0003383587,0.0002849119,0.001733089,0.00004201701,0.00003835048,0.000004016928,0.00006867853,0.0004942836,0.9871193,0.00003848882,0.009576056,0.0002624754],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9821886,0.0006319135,0.01095215,0.0002729455,0.00009513416,0.0006157351,0.00002951413,0.00006108119,0.005152951],"genre_scores_gemma":[0.9925007,0.00009455905,0.005485704,0.0001417136,0.0004437233,0.0001029042,0.00007954164,0.00004029294,0.001110871],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01031211,"threshold_uncertainty_score":0.7841196,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01804525486363868,"score_gpt":0.2554331517773513,"score_spread":0.2373878969137126,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}