{"id":"W1970617888","doi":"10.1371/journal.pone.0109213","title":"Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage","year":2014,"lang":"en","type":"article","venue":"PLoS ONE","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":119,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research","keywords":"CRISPR; Cas9; Genome editing; Nuclease; Biology; Computational biology; Palindrome; Guide RNA; Chromatin; DNA; In silico; Genetics; RNA; Subgenomic mRNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001581565,0.0003066468,0.0003134992,0.0001716136,0.0001535699,0.0004638979,0.0002431953,0.0003775816,0.00219685],"category_scores_gemma":[0.000397944,0.000180068,0.0002658498,0.0001180883,0.0002185667,0.0002101041,0.0004699563,0.0004489659,0.0008816385],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003096006,"about_ca_system_score_gemma":0.0001939604,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005216182,"about_ca_topic_score_gemma":0.0009463216,"domain_scores_codex":[0.9996991,0.00003645223,0.00001509579,0.00009438045,0.00009367893,0.00006124608],"domain_scores_gemma":[0.9997157,0.000106258,0.00006105977,0.00003661544,0.00002452444,0.00005582283],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005680801,0.00001886105,0.0006349519,0.00002251948,0.000004820918,0.0000471479,0.00001220625,0.0001391759,0.9972762,0.0001641161,0.00004776507,0.001575421],"study_design_scores_gemma":[0.000004515476,0.00007311997,0.005021155,0.000004753129,0.000005807804,0.0001645989,0.00002785601,0.001856498,0.9912389,0.0001909763,0.001407467,0.000004371469],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9846823,0.0004732672,0.01034722,0.00005396395,0.00002743464,0.00002190006,0.0002762107,0.0002683253,0.003849358],"genre_scores_gemma":[0.9944199,0.0001103454,0.003727001,0.00004529321,0.000003118057,0.000006945085,0.0002013964,0.00002559611,0.001460439],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00219685,"threshold_uncertainty_score":0.007349193,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01804525486363868,"score_gpt":0.2554331517773513,"score_spread":0.2373878969137126,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}