{"id":"W1971008570","doi":"10.1093/nar/gkm010","title":"DNA supercoiling suppresses real-time PCR: a new approach to the quantification of mitochondrial DNA damage and repair","year":2007,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"Mitochondrial Function and Pathology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":98,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University Health Centre","funders":"McGill University Health Centre; McGill University","keywords":"DNA supercoil; Biology; Mitochondrial DNA; DNA; DNA damage; Molecular biology; Real-time polymerase chain reaction; Nucleic acid; DNA repair; Denaturation (fissile materials); Nuclear DNA; Biophysics; Biochemistry; Gene; Chemistry; DNA replication","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001661896,0.0001316183,0.0001752416,0.0001471946,0.0002070689,0.00004008022,0.0003506361,0.000243563,0.0001159766],"category_scores_gemma":[0.0009018757,0.0001035093,0.00008367971,0.0003379317,0.0002291592,0.000008307637,0.0002493944,0.0002243979,0.00007022268],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000015197,"about_ca_system_score_gemma":0.0001347814,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001171932,"about_ca_topic_score_gemma":0.00005230553,"domain_scores_codex":[0.9979921,0.000323257,0.0003069682,0.0005439904,0.0004244586,0.0004092236],"domain_scores_gemma":[0.9985989,0.0001346516,0.0000429863,0.0007744718,0.0002537488,0.0001951962],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006031553,0.0000663725,0.0002651174,0.00002636863,0.00002279281,0.000001816582,0.0003996879,0.00001548538,0.9528981,0.0004850442,0.04431962,0.0008964122],"study_design_scores_gemma":[0.0008911033,0.0006306344,0.003368762,0.00002001534,0.00002116595,0.00003575937,0.001061947,0.0003759594,0.7700204,0.00009016208,0.2232512,0.0002329432],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9776405,0.0001188307,0.0061174,0.000609347,0.0002823616,0.0006559937,0.00001920212,0.00003264643,0.01452371],"genre_scores_gemma":[0.9453011,0.0004240392,0.02946223,0.0002742099,0.002785163,0.00005793479,0.0002269698,0.00008706602,0.02138133],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1828778,"threshold_uncertainty_score":0.4220988,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04982678741457053,"score_gpt":0.330335572406247,"score_spread":0.2805087849916765,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}