{"id":"W1971778847","doi":"10.1038/nmeth.1207","title":"Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors","year":2008,"lang":"en","type":"article","venue":"Nature Methods","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":352,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Förster resonance energy transfer; Aequorea victoria; Biosensor; Fluorescence; Green fluorescent protein; Fluorescent protein; Biophysics; Chemistry; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002941715,0.001725104,0.002056955,0.001255133,0.0006475448,0.001904159,0.004855583,0.002995608,0.003960874],"category_scores_gemma":[0.002709091,0.001647957,0.000921629,0.0009930085,0.0008606747,0.002580079,0.00233736,0.004273438,0.003837272],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001709523,"about_ca_system_score_gemma":0.0003797243,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002076359,"about_ca_topic_score_gemma":0.0003897738,"domain_scores_codex":[0.9967122,0.0007762944,0.0001698606,0.0008463806,0.001133734,0.0003614304],"domain_scores_gemma":[0.9991949,0.0002761395,0.0001111165,0.0001513183,0.0001587804,0.0001077078],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000260457,0.00009802768,0.0001020602,0.0002044047,0.00003481465,0.0001097234,0.00009120096,0.0006680762,0.9711905,0.006433223,0.001720954,0.0190865],"study_design_scores_gemma":[0.00002163299,0.00005785723,0.00007558971,0.000008365609,0.00001543187,0.0002399227,0.00001236551,0.006928151,0.9883045,0.0007035052,0.003612015,0.00002060273],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.153021,0.003352165,0.8285829,0.001362386,0.0005219472,0.0003503523,0.000664413,0.003982331,0.008162406],"genre_scores_gemma":[0.4996016,0.002262888,0.4839139,0.0008756859,0.0001542748,0.000952818,0.001086317,0.0007998298,0.01035283],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004855583,"threshold_uncertainty_score":0.01555747,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0149252127741819,"score_gpt":0.3687402437413047,"score_spread":0.3538150309671227,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}