{"id":"W1971801782","doi":"10.1016/s0022-2836(03)00762-9","title":"Human 20α–Hydroxysteroid Dehydrogenase: Crystallographic and Site-directed Mutagenesis Studies Lead to the Identification of an Alternative Binding Site for C21-steroids","year":2003,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"Aldose Reductase and Taurine","field":"Biochemistry, Genetics and Molecular Biology","cited_by":76,"is_retracted":false,"has_abstract":false,"ca_institutions":"Wilfrid Laurier University; Université Laval","funders":"Medical Research Council Canada; Fondation de l’Université Laval; Hydro-Québec; Ministry of Higher Education and Scientific Research","keywords":"Chemistry; Cofactor; Hydroxysteroid dehydrogenase; Dehydrogenase; Stereochemistry; Mutagenesis; Binding site; Ternary complex; Active site; Steroid; Hydrogen bond; Oxidoreductase; Site-directed mutagenesis; Enzyme; Biochemistry; Mutation; Molecule; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007414536,0.0001792508,0.000322083,0.000194113,0.0001249897,0.00001756529,0.0001892471,0.000119897,0.000001619181],"category_scores_gemma":[0.0002350782,0.0001317734,0.0001570295,0.0001570079,0.0001501151,0.000009549517,0.00006325339,0.00008306622,7.54971e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001469634,"about_ca_system_score_gemma":0.00003536832,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007524787,"about_ca_topic_score_gemma":0.00004346322,"domain_scores_codex":[0.9985056,0.0003169368,0.0005838585,0.000276242,0.0001038372,0.0002135053],"domain_scores_gemma":[0.9987217,0.00002651351,0.0005374215,0.000271709,0.0003608444,0.00008183231],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000745072,0.00006535657,0.0005102563,0.00002739089,0.0004424486,0.00001224928,0.0001941314,0.0001742905,0.9979813,0.00007306484,0.00007405261,0.0003709698],"study_design_scores_gemma":[0.0007788458,0.001460741,0.0002517479,0.00002527161,0.00013999,0.0002000989,0.000247488,0.00003806654,0.994413,0.0002754508,0.002016197,0.000153157],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9898434,0.006228912,0.003282584,0.0001311868,0.0001473348,0.0002858028,0.00006971913,0.000004261151,0.000006841159],"genre_scores_gemma":[0.9981354,0.0003839486,0.001075424,0.0001263373,0.000113944,0.000018267,0.00007383683,0.00002241658,0.00005043319],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008292034,"threshold_uncertainty_score":0.5373563,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01768103509878863,"score_gpt":0.3193257739087605,"score_spread":0.3016447388099719,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}