{"id":"W1971801782","doi":"10.1016/s0022-2836(03)00762-9","title":"Human 20α–Hydroxysteroid Dehydrogenase: Crystallographic and Site-directed Mutagenesis Studies Lead to the Identification of an Alternative Binding Site for C21-steroids","year":2003,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"Aldose Reductase and Taurine","field":"Biochemistry, Genetics and Molecular Biology","cited_by":76,"is_retracted":false,"has_abstract":false,"ca_institutions":"Wilfrid Laurier University; Université Laval","funders":"Medical Research Council Canada; Fondation de l’Université Laval; Hydro-Québec; Ministry of Higher Education and Scientific Research","keywords":"Chemistry; Cofactor; Hydroxysteroid dehydrogenase; Dehydrogenase; Stereochemistry; Mutagenesis; Binding site; Ternary complex; Active site; Steroid; Hydrogen bond; Oxidoreductase; Site-directed mutagenesis; Enzyme; Biochemistry; Mutation; Molecule; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005640629,0.0007777732,0.0007065173,0.0002334493,0.0004474796,0.0009217001,0.0009511273,0.0009886689,0.001139185],"category_scores_gemma":[0.0004304368,0.0002714003,0.0005608909,0.0003490893,0.000454298,0.0002781163,0.0002442009,0.0009499277,0.0006873741],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001452436,"about_ca_system_score_gemma":0.0007648982,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004381036,"about_ca_topic_score_gemma":0.004658949,"domain_scores_codex":[0.9997737,0.00004747386,0.00002369042,0.00003799449,0.00008277504,0.00003435181],"domain_scores_gemma":[0.9997253,0.00005030492,0.00005103818,0.00002883572,0.00005395911,0.00009057485],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00234246,0.0002859896,0.002112041,0.0001635038,0.00009807324,0.001182244,0.0003180259,0.002498953,0.9810245,0.001868123,0.001175133,0.006930831],"study_design_scores_gemma":[0.0002880782,0.0002537045,0.004985034,0.00002498249,0.0001637179,0.002196227,0.0001786081,0.007213951,0.9656953,0.0005099391,0.01842676,0.00006379021],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9873426,0.002350428,0.006900812,0.0006557697,0.0001703801,0.00007402706,0.0007191907,0.0001803281,0.001606328],"genre_scores_gemma":[0.981986,0.001737539,0.01129508,0.0002101867,0.00004308133,0.00002949013,0.002444622,0.00005929849,0.0021947],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004381036,"threshold_uncertainty_score":0.01053816,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01768103509878863,"score_gpt":0.3193257739087605,"score_spread":0.3016447388099719,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}